| 小麦高代材料畅-99-1的分子生物学研究 |
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| 引用本文： | 王创云,畅志坚,刘建霞.小麦高代材料畅-99-1的分子生物学研究[J].山西农业科学,2008,36(2):13-14. |
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| 作者姓名： | 王创云 畅志坚 刘建霞 |
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| 作者单位： | 1. 山西省农业科学院作物遗传研究所,山西,太原,030031
2. 山西大学生物工程学院,山西,太原,030031 |
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| 摘 要： | 实验以利用八倍体小黑麦和普通小麦远缘杂交后代中选育出的一个品系畅-99-1为供试材料,在分子生物学的基础上,利用特异PCR技术等对畅-99-1品系作了较为系统的初步研究,发现了该品系中特异的外源DNA片断,为远缘杂交创造育种材料的方法提供一个可行的依据,结果显示:根据黑麦的特异重复序列psc119.1,pscH20设计引物,对畅-99-1品系进行扩增,psc119.1,pscH20能特异的扩增出黑麦的特异条带,确定畅-99-1为黑麦小麦小片段易位系。
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| 关 键 词： | 小麦 黑麦 小片段易位 PCR扩增 |
Study on the Wheat Matierial Chang 99-1 Based on Molecular Biology |
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| WANG Chuang-yun,CHANG Zhi-jian,LIU Jian-xia.Study on the Wheat Matierial Chang 99-1 Based on Molecular Biology[J].Journal of Shanxi Agricultural Sciences,2008,36(2):13-14. |
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| Authors: | WANG Chuang-yun CHANG Zhi-jian LIU Jian-xia |
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| Abstract: | Advanced wheat cultivator Chang99-1 whose parents are common wheat(Triticum aestivum) and rye(Secale cereals) types named TAI7047 derived from Secale cereals.Using the PCR studied Chang99-1 cultivator systematically.The author verified the feasibility of the breeding method.According the specific repeat sequence in the genome of Secale cereals,the designed primers of specific repeated sequence from Secale cereals amplified the Chang99-1,the results showed that psc119.1,pscH20 could amplified the products correctly. |
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| Keywords: | Wheat Black wheat Small piece transposition PCR increases |
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