| 产志贺毒素EHEC国内分离株Stx2基因的克隆及生物信息学分析 |
| |
| 引用本文： | 唐中伟,周志江.产志贺毒素EHEC国内分离株Stx2基因的克隆及生物信息学分析[J].山西农业科学,2011,39(2):167-169. |
| |
| 作者姓名： | 唐中伟 周志江 |
| |
| 作者单位： | 1. 山西农业大学生命科学学院,山西太谷,030801
2. 天津大学农业与生物工程学院,天津,300072 |
| |
| 基金项目： | 山西省回国留学人员科研资助项目(2008078) |
| |
| 摘 要： | 通过对来源于人和牛粪的EHEC的Stx2基因的克隆,并利用生物信息学方法对3株菌Stx2基因的序列进行分析,结果表明:EHEC94H的Stx2序列与标准株Stx2,Stx2c,Stx2d核苷酸序列同源分别为99.8%,99.5%,90.9%,EHEC 042的Stx2序列与标准株Stx2,Stx2c,Stx2d核苷酸序列同源性分别为98.9%,98.9%,90.5%,EHEC 094的Stx2序列与标准株Stx2,Stx2c,Stx2d核苷酸序列同源性分别为97.9%,98.0%,87.1%。
|
| 关 键 词： | EHEC Stx2 克隆 序列分析 生物信息学分析 |
Cloning and Bioinformatics Analysis of SW Shiga Toxin-producing EHEC Strains Isolated from China |
| |
| TANG Zhong-wei,ZHOU Zhi-jiang.Cloning and Bioinformatics Analysis of SW Shiga Toxin-producing EHEC Strains Isolated from China[J].Journal of Shanxi Agricultural Sciences,2011,39(2):167-169. |
| |
| Authors: | TANG Zhong-wei ZHOU Zhi-jiang |
| |
| Institution: | TANG Zhong-wei1,ZHOU Zhi-jiang2(1.College of Life Sciences,Shanxi Agricultrual University,Taigu 030801,China,2.School of Agriculture and Bioengineering,Tianjin University,Tianjin 300072,China) |
| |
| Abstract: | The Stx2 gene of EHEC from cow and human dung were cloned and 3 bacteria Stx2 gene sequences were analyzed with bioinformatics methods.The results showed that: the similarity of Stx2 in EHEC 94H was 89%,85%and 84%,compared with corresponding sequences in Stx2,Stx2c and Stx2d,respectively;the similarity of Stx2 in EHEC 042 was 98.9%,98.9%,90.5% compared with corresponding sequences in Stx2,Stx2c and Stx2d respectively;and the similarity of Stx2 in EHEC 094 was 97.9%,98.0%,87.1% compared with corresponding se... |
| |
| Keywords: | EHEC Stx2 cloning bioinformatics analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
