生长素和细胞分裂素在马铃薯愈伤组织分化中的作用

以晋薯7号马铃薯茎尖、茎段、叶圆片为外植体,通过愈伤组织的诱导和根、不定芽的分化,研究生长素和细胞分裂素不同配比在马铃薯愈伤组织分化中的作用。试验结果表明:6-BA 1.5 mg/L+NAA 1.0mg/L+3%蔗糖+0.6%琼脂有利于茎段愈伤组织的生长,MS+6-BA 2.0 mg/L+ZT 2.0 mg/L+NAA 0.5mg/L+GA35.0 mg/L+3%蔗糖+0.6%琼脂有利于茎尖不定芽的分化,MS+6-BA 1.5 mg/L+NAA 0.5mg/L+GA35.0 mg/L+3%蔗糖+0.6%琼脂对叶片根的分化效果较好。

The Function of Growth Hormone and Cell Division Hormone in Potato Healing Tissue Differentiation

Study on function of proportion between growth hormone and cell division hormone in potato healing tissue differentiation has been done through healing tissue induction and differentiation of root and irregular bud,with stem point,stem section and leaf blade of "Jin Shu No.7"potato being outer plant frame.The result indicated that the agar with the formula 6-BA 1.5 mg/L+NAA 1.0 mg/L+3% sucrose +0.6% is good for healing tissue growth of stem section,and the agar of 6-BA 1.5 mg/L+NAA 1.0 mg/L+3% sucrose +0.6% is in favor of differentiation of irregular bud of stem point,and agar of MS+6-BA 1.5 mg/L+NAA 0.5 mg/L+GA3 5.0 mg/L+3% sucrose +0.6% has a good effect on differentiation of leaf root.