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樱桃叶绿素含量测定方法研究
引用本文:孙俊宝,王建新.樱桃叶绿素含量测定方法研究[J].山西农业科学,2010,38(3):18-19,33.
作者姓名:孙俊宝  王建新
作者单位:1. 山西省农业科学院果树研究所,山西,太谷,030815
2. 山西省农业科学院现代农业研究中心,山西,太原,030031
摘    要:以"龙田晚红"樱桃叶片为试材,通过采用不同的有机试剂作为提取液,在不同提取时间下进行叶绿素含量的测定。结果表明,用1∶1的80%丙酮和95%乙醇混合液提取的叶绿素含量最高,可达3.848 mg/g,显著高于其他有机试剂,用95%乙醇提取的叶绿素含量最低,为2.682 mg/g;浸提14 h时,叶绿素含量最高,16 h后含量趋于稳定。

关 键 词:樱桃  叶绿素  混合液

Study on Method for Determination of Chlorophyll in Cherry Leaves
SUN Jun-bao,WANG Jian-xin.Study on Method for Determination of Chlorophyll in Cherry Leaves[J].Journal of Shanxi Agricultural Sciences,2010,38(3):18-19,33.
Authors:SUN Jun-bao  WANG Jian-xin
Institution:SUN Jun-bao1,WANG Jian-xin2(1.Pomology Institute,Shanxi Academy of Agricultural Sciences,Taigu 030815,China,2.Modern Agriculture Research Center,Taiyuan 030031,China)
Abstract:Several organic reagents and the mixed of them were used in the experiment to extract chlorophyll from cherry leaves of Longtianwanhong at various time.The results showed that the mixed reagent of 80% acetone with 95% ethyl alcohol in ratio of 1:1 had the best result and the extracted chlorophyll was as high as 3.848mg/g,which Was significantly higher than with the other organic reagents.With 95% ethyl alcohol the extracted chlorophyll Was the lowest of 2.682 mg/g; 14 h's extraction had the highest extracted chlorophyll and after 16 h's extraction,the extracted chlorophyll would keep stable.
Keywords:Cherry  Chlorophyll  Mixed reagent
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