| 哈茨木霉液体发酵产厚垣孢子条件的优化 |
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| 引用本文： | 粟静,罗志威,吕黎,王蕾,丰来.哈茨木霉液体发酵产厚垣孢子条件的优化[J].山西农业科学,2014(2):169-173. |
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| 作者姓名： | 粟静 罗志威 吕黎 王蕾 丰来 |
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| 作者单位： | 湖南泰谷生物科技股份有限公司,农业部植物营养与生物肥料重点实验室,湖南长沙410205 |
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| 基金项目： | 新型生物有机药肥生产关键技术产业化（2013NK4009） |
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| 摘 要： | 在单因素试验结果的基础上,进一步应用Box-Behnken中心组合试验和响应面分析法优化发酵条件。结果表明,最佳发酵条件为:玉米粉1.01%,酵母膏0.47%,(NH4)2SO40.05%,K2HPO40.06%,MgSO40.05%,pH值5.79,28℃培养。研究结果为哈茨木霉厚垣孢子的大规模发酵生产提供了一定的技术支持。
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| 关 键 词： | 哈茨木霉 厚垣孢子 响应面法 液体发酵 |
Optimization of the Liquid Fermentation Conditions of Trichoderma harzianum Chlamydospores |
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| SU Jing,LUO Zhi-wei,LYU Li,WANG Lei,FENG Lai.Optimization of the Liquid Fermentation Conditions of Trichoderma harzianum Chlamydospores[J].Journal of Shanxi Agricultural Sciences,2014(2):169-173. |
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| Authors: | SU Jing LUO Zhi-wei LYU Li WANG Lei FENG Lai |
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| Institution: | ( Key Laboratory of Plant Nutrition ＆ Biological Fertilizer, Ministry of Agriculture, P.R. China, Hunan Taigu Bio-Tech Co.Ltd, Changsha 410205, China) |
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| Abstract: | Based on single-factor experiments, we applied Box-Behnken central composite experiments and response surface analysis to optimize the liquid fermentation conditions. The results showed that the optimum fermentation conditions were the following： corn flour 1.01%, yeast extract 0.47%, （NH4）2SO4 0.05%, K2HPO4 0.06%, MgSO4 0.05%, pH 5.79 and 28 ℃. The study provided a technical suppor for large scale fermentation of Trichoderma harzianum chlamydospores.Based on single-factor experiments, we applied Box-Behnken central composite experiments and response surface analysis to optimize the liquid fermentation conditions. The results showed that the optimum fermentation conditions were the following： corn flour 1.01%, yeast extract 0.47%, （NH4）2SO4 0.05%, K2HPO4 0.06%, MgSO4 0.05%, pH 5.79 and 28 ℃. The study provided a technical suppor for large scale fermentation of Trichoderma harzianum chlamydospores. |
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| Keywords: | Trichoderma harzianum chlamydospores response surface method the liquid fermentation |
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