| 白羊草茎段愈伤组织诱导和植株再生 |
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| 引用本文： | 于娜,董宽虎.白羊草茎段愈伤组织诱导和植株再生[J].山西农业科学,2012,40(6):575-578. |
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| 作者姓名： | 于娜 董宽虎 |
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| 作者单位： | 1. 山西省林业职业技术学院园林系,山西太原,030009
2. 山西农业大学动物科技学院,山西太谷,030801 |
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| 摘 要： | 首次采用白羊草拔节期茎段为外植体,通过对其组织培养中愈伤组织诱导和植株再生情况进行研究,从而筛选出其在组织培养过程中较合适的激素组合。结果表明,茎段作为外植体时,愈伤诱导在仅附加2,4-D 2.0 mg/L诱导培养基中的诱导率高于2,4-D和6-BA的激素组合;诱导出的愈伤组织转入MSB+6-BA 0.07 mg/L+NAA 0.05 mg/L分化培养基中,分化率显著高于其他激素组合。
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| 关 键 词： | 白羊草 茎段 愈伤诱导 植株再生 |
Callus Induction and Plant Regeneration from Stem Segments of Bothriochloa ischaemum |
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| YU Na
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DONG Kuan-hu.Callus Induction and Plant Regeneration from Stem Segments of Bothriochloa ischaemum[J].Journal of Shanxi Agricultural Sciences,2012,40(6):575-578. |
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| Authors: | YU Na DONG Kuan-hu |
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| Institution: | 1.Department Gardens,Shanxi Vocational and Technical College of Forestry,Taiyuan 030009,China; 2.College of Animal Science & Technology,Shanxi Agricultural University,Taigu 030801,China) |
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| Abstract: | Stem segment of Bothriochloa ischaemum as explant,callus induction and planets regeneration was conducted to screen a suitable hormone combination.Results showed that the inductivity of Bothriochloa ischaemum stem segment was higher in MSB medium added only 2.0 mg/L 2,4-D than in hormone combination of 2,4-D and 6-BA.The embryo calluses obtained from induction medium could be succeeded on medium MSB+6-BA 0.07 mg/L+NAA 0.05 mg/L,they had remarkable high differentiation frequency than in other hormone combinations. |
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| Keywords: | Bothriochloa ischaemum stem segment embryogenic callus plant regeneration |
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