火龙果茎段组织培养快繁技术研究

以火龙果的幼嫩茎段为外植体进行组培快繁研究,结果表明,火龙果不同长度的茎段均能产生愈伤组织,茎上的不定芽大部分可以启动生长,其中,以3 cm长的幼嫩茎段诱导效果最佳,愈伤组织诱导率为100%;不定芽诱导培养基以MS+6-BA 4 mg/L+NAA 0.1 mg/L效果最好,诱导率可达75%;当6-BA质量浓度为8 mg/L和NAA质量浓度为0.05 mg/L时,不定芽的繁殖系数最高,当6-BA质量浓度为1 mg/L和NAA质量浓度为0.2 mg/L时,虽然不定芽的繁殖系数低,但其不定芽生长较快、苗壮,可以依生产的需要交替使用这2种培养基;适宜的生根培养基为1/2 MS+IBA2.0 mg/L。

Tissue Culture and Rapid Propagation of Hylocereus undatus Stem

Tissue culture and rapid propagation of Hylocereus undatus young stem were studied.The results showed: stems of different length could be induced and produced callus,and most adventitious buds could be initiated to grow,in which the best callus inductivity for 3 cm length stem reached 100%;The best culture medium of adventitious buds induction was MS+6-BA 4.0 mg/L+NAA 0.1 mg/L on which inductivity reached 75%;The propagation ratio of adventitious buds was the highest when concentration of 6-BA was 8 mg/L and NAA 0.05 mg/L,but the growth of adventitious buds was the fastest at concentration of 1 mg/L 6-BA and 0.2 mg/L NAA.So we often use these two mediums alternately according to demands;The best rooting culture medium was 1/2 MS+IBA 2.0 mg/L.