| SSR分子标记技术在玉米杂种优势群划分中的应用 |
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| 引用本文： | 骈跃斌,许晶,武岩军,王华,古晓红.SSR分子标记技术在玉米杂种优势群划分中的应用[J].山西农业科学,2012,40(5):439-441,444. |
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| 作者姓名： | 骈跃斌 许晶 武岩军 王华 古晓红 |
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| 作者单位： | 1. 山西省农业科学院食用菌研究所,山西太原,030031
2. 山西省农业科学院作物科学研究所,山西太原,030032 |
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| 基金项目： | 山西省农业科学院育种工程项目 |
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| 摘 要： | 利用SSR分子标记研究了32份玉米自交系的遗传变异,初步进行了杂种优势群划分。从40对SSR引物中筛选出24对扩增产物具有稳定多态性的引物,24对引物在供试材料中共检出165个等位基因变异,每对引物检测到等位基因3～12个,平均6.875个。按UPGMA方法进行的聚类分析结果表明,供试自交系可划分为4个类群,利用SSR标记可以对玉米自交系的遗传变异进行初步分析,并可用于杂种优势群划分。
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| 关 键 词： | 玉米 SSR分子标记 自交系 聚类分析:杂种优势群 |
Application of SSR Markers to Heterotic Grouping of Maize Inbred Lines |
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| PIAN Yue-bin
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XU Jing
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WU Yan-jun
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WANG Hua
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GU Xiao-hong.Application of SSR Markers to Heterotic Grouping of Maize Inbred Lines[J].Journal of Shanxi Agricultural Sciences,2012,40(5):439-441,444. |
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| Authors: | PIAN Yue-bin XU Jing WU Yan-jun WANG Hua GU Xiao-hong |
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| Institution: | 1.Institute of Edible Fungi,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China; 2.Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China) |
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| Abstract: | Simple sequence repeats(SSRs) were used to detect genetic variation among 32 maize inbred lines.24 SSR primers selected from 40 primers gave stable profiles amplified in sample of 32 inbred lines.24 primers produced 165 polymorphic amplified fragments.The average number of alleles per SSR locus was 6.875 in a range from 3 to 12.The cluster analysis showed that the inbred lines could be classified into four groups and SSR makers could be used in genetic variation measurement and heterotic groups assignment. |
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| Keywords: | maize SSR markers inbred line cluster analysis heterotic group |
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