不同激素对补血草器官分化的影响

以补血草的带节花梗为材料,在离体条件下研究不同激素对花梗愈伤组织的诱导、不定芽分化、继代培养以及生根的影响,建立和优化离体再生体系,为补血草快速繁殖、种质资源保存以及遗传转化提供有效的途径和技术。结果表明,MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L为补血草花梗愈伤组织诱导的最佳培养基,愈伤组织诱导率最高(89.2%),出愈速度最快(12 d),产生的愈伤组织颜色翠绿,质地紧密;不定芽诱导以MS+6-BA 2.0 mg/L+NAA 0.1 mg/L的效果最佳,诱导率为74.3%,平均每块愈伤组织诱导的不定芽数为3.6个;在NAA 0.1～0.2 mg/L+6-BA 1.5～2.0 mg/L时的继代增殖效果最好,分化率大于85%,使幼苗数增加3倍以上;生根培养以1/2 MS+IBA 1.0 mg/L+蔗糖20 g/L时的生根率最大,为100%。

Effcet of Hormons on Organogenesis of Limonium sinuatum

In vitro culture and rapid propagation of Limonium sinuatum,an important medicinal herb,was achieved by culturing peduncle-node explants.MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L was optimized for callus induction.The percentage of callus forming adventitious shoots and the average number of adventitious buds produced per callus were related with the concentrations of auxin and cytokinin.MS medium supplemented with 2.0 mg/L N6-Benzyladenine(BA) and 0.1 mg/L α-Naphthaleneacetic acid(NAA) supported induction of adventitious shoots from inflorescence-node callus.Adventitious shoots were multiplied by subculturing on MS medium supplemented with BA 1.5～2.0 mg/L in combination with NAA 0.1～0.2 mg/L and the multiplication rate was above 85%.Shoots were rooted on 1/2 MS basal medium with 1.0mg/L indole-3-butyric acid(IBA) and 20 g sucrose,and the rooting rate could by 100%.