| 两种物理方法破碎大肠杆菌比较试验 |
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| 引用本文： | 薛俊龙,王采先,詹丽娥,张李俊,田林君,李红丽,侯明全.两种物理方法破碎大肠杆菌比较试验[J].山西农业科学,2005,33(4):77-79. |
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| 作者姓名： | 薛俊龙 王采先 詹丽娥 张李俊 田林君 李红丽 侯明全 |
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| 作者单位： | 1. 山西省农业科学院畜牧兽医研究所,山西,太原,030032
2. 运城市畜牧发展中心,山西,运城,044000 |
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| 基金项目： | 山西省科技攻关项目(041050) |
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| 摘 要： | 采用超声波和反复冻融两种物理方法,对一定浓度的标准致病性大肠杆菌进行破碎率比较试验。结果表明:在试验条件下,超声波破碎法达到97%以上的破碎率所需时间为30~50min,尤其是菌液浓度为每毫升50亿,用Ф10变幅杆破碎,30min破碎率可达99.9%。而反复冻融破碎,无论液氮的低温冷冻人为速溶或自溶,还是采取冷冻箱的冻融,达到50%以上的破碎,要耗时数小时,且要经过预冷冻的过程。
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| 关 键 词： | 大肠杆菌 超声波破碎 冷冻破碎 |
| 文章编号： | 1002-2481(2005)04-0077-03 |
| 收稿时间： | 2005-06-08 |
| 修稿时间： | 2005年6月8日 |
A Comparison of Two Physical Methods to Break E.coli Germs |
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| XUE Jun-long,WANG Cai-xian,ZHAN Li-e,ZHANG Li-jun,TIAN Lin-jun,LI Hong-li,HOU Ming-quan.A Comparison of Two Physical Methods to Break E.coli Germs[J].Journal of Shanxi Agricultural Sciences,2005,33(4):77-79. |
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| Authors: | XUE Jun-long WANG Cai-xian ZHAN Li-e ZHANG Li-jun TIAN Lin-jun LI Hong-li HOU Ming-quan |
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| Institution: | 1. Institute of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Science , Taiyuan 030032, China ; 2. Center of Animal Husbandry and Veterinary in Yuncheng , Yuncheng 043000, China |
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| Abstract: | Both physical methods ultrasonic wave and repeated freeze-melt were used to break E.coli germs.The results show:it took 30～50 minutes to break the germs in fluid containing 5～10 billion germs/ml by ultrasonic wave instrument with variable amplitude lever Φ6 and when fluid germ concentration was 50 billion/ml it took 30 min.With Φ10 instrument for germs to be broken up to 99.9%.While the method of repeat freeze-melt,hours time and pre-freeze were needed for over 50% germs could be broken,no matter artificial or self melting. |
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| Keywords: | E coli germ Ultrasonic wave breaking Frozen-breaking |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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