影响普那菊苣高效再生相关因素的研究

以普纳菊苣叶片为外植体,通过培养基优化、抗生素敏感性试验等对其再生体系进行了研究。结果表明,MS+6-BA1.5mg/L+IBA0.2mg/L+AgNO30.5mg/L+Vc 0.3mg/L为诱导愈伤形成和芽再生的最佳培养基,诱导率为100%;MS+NAA1.5mg/L为最适生根培养基,生根率为98%。卡那霉素和头孢霉素添加实验表明,26mg/L卡那霉素是普那菊苣苗能够存活的上限;500～750mg/L头孢霉素是抑制农杆菌过度生长的最适浓度范围。研究初步建立了普纳菊苣的高效再生体系,为进一步对其进行遗传转化研究奠定了基础。

Study on Factors of Affecting High Efficiency Regeneration of Cichorium intybus

The regeneration system of Cichorium intybus L.cv.Puna,using its leaves as explants,was studied by optimizing phytohormone combinations and antibiotics concentrations in the media.The results of medium selection suggested that the medium for callus induction and shoot regeneration was MS+6-BA1.5mg/L+IBA 0.2mg/L+AgNO30.5mg/L+Vc0.3mg/Land the regeneration frequency was up to 100%.The optimum medium for rooting was MS + NAA 1.5 mg/L,and the rooting frequency was 98%.The results of antibiotic experiment showed that Kanamycin concentration up to 26 mg/L was the maximum amount for the survival of chicory plantlets;the growth of Agrobacterium strain can be inhibited completely when Cefazolin sodium concentration was between 500～750mg/L.In this study the effective regeneration system of Cichorium intybus was established which laid a solid foundation for further study of genetic transformation of Cichorium intybus.