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| 农杆菌介导抗草甘膦基因(EPSPS)的玉米转化及相关因子的影响研究 | |
| 引用本文： | 王秀红,白建荣,孙毅,史向远,任志强.农杆菌介导抗草甘膦基因(EPSPS)的玉米转化及相关因子的影响研究[J].山西农业科学,2010,38(1):11-14,18. |
| 作者姓名： | 王秀红 白建荣 孙毅 史向远 任志强 |
| 作者单位： | 1. 山西省农业科学院现代农业研究中心,山西,太原,030031 2. 山西省农业科学院作物遗传研究所,山西,太原,030031 3. 山西省农业科学院农业生物技术研究中心,山西,太原,030031 |
| 基金项目： | 山西省国际科技合作项目 |
| 摘 要： | 对影响农杆菌转化效率的一些因素进行了研究优化,结果表明,诱导培养基中添加200 mg/L的头孢霉素既能抑制农杆菌的生长又不影响幼胚的愈伤诱导;诱导和筛选培养基中添加5 mg/L硝酸银能有效抑制玉米愈伤组织褐变,显著改善愈伤组织及胚性愈伤组织的诱导率;分化培养基中添加2μmol/L的嘧啶醇能延缓试管苗的生长速度,使苗更健壮。利用优化的根癌农杆菌介导转化体系将抗草甘膦基因(EPSPS)转入玉米杂交材料HiⅡ中,获得了PCR阳性植株。 |
| 关 键 词： | 玉米 农杆菌介导 抗草甘膦基因(EPSPS) 相关因子 |
Study on Agrobacterium tumefaciens-mediated Glyphosate-resistant Gene(EPSPS) Transformation and Correlation Factors in Maize |
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| WANG Xiu-hong,BAI Jian-rong,SUN Yi,SHI Xiang-yuan,REN Zhi-qiang.Study on Agrobacterium tumefaciens-mediated Glyphosate-resistant Gene(EPSPS) Transformation and Correlation Factors in Maize[J].Journal of Shanxi Agricultural Sciences,2010,38(1):11-14,18. | |
| Authors: | WANG Xiu-hong BAI Jian-rong SUN Yi SHI Xiang-yuan REN Zhi-qiang |
| Institution: | WANG Xiu-hong1,BAI Jian-rong2,SUN Yi3,SHI Xiang-yuan1,REN Zhi-qiang2(1.Modern Agricultural Research Center,Shanxi Academy of Agriculturd Sciences,Taiyuan 030031,China,2.Institute of Crop Genetics,Shanxi Academy,Agricultural Sciences,3.Agricultural Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,China) |
| Abstract: | Several factors influencing transformation efficiency were studied and optimized.It showed that,adding 200 mg/L Cofotaxime could inhibit the growth of Agrobacterium but not influence the callus induction;adding 5 mg/L AgNO_3 could inhibit maize callus browning,the regeneratet maize plantlets grew more slowly and robuster on the medium with 2μmol/L ancymidol.The glyphosate-resistant gene (EPSPS) was introduced into Hi II,a maize hybrid,by Agrobacterium tumefaciens method.The PCR positive plants were obtained. |
| Keywords: | Maize Agrobacterium tumefaciens-mediated transformation Glyphosate-resistant gene (EPSPS) Correlation factors |
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