东北点地梅下胚轴再生体系的建立

为保存野生资源和满足需要,以东北点地梅的下胚轴为材料,采用组织培养的方法进行了愈伤组织诱导与分化、不定芽生根、试管苗的生根、生根继代及移栽和定植的研究。结果表明,MS+ZT0.2 mg/L+6-BA0.1 mg/L+2,4-D2.1 mg/L是下胚轴愈伤组织诱导培养的理想培养基;MS+NAA0.1 mg/L+ZT0.6 mg/L是愈伤组织分化培养和不定芽分化继代增殖培养的理想培养基;用10 mg/L的IAA溶液对不定芽处理24 h,将不定芽接种到1/2 MS培养基上生根是不定芽生根培养的理想方法。在温室中试管苗移栽成活率为94.1%,定植成活率为98.5%,定植成活的试管苗保持了野生东北点地梅的生物性状。

Establishment of Regeneration Clone from Hypocotyl of Androsace filiformia

To preserve the wild resources and meet the needs of people,with the method of tissue culture,hypocotyl of Androsace filiformia were used as material to do the research on callus inducement and differentiation,rooting of adventitious buds,rooting and rooting multiplication of tube seedlings,transplantation of tube seedlings and stable planting.The results showed that the ideal medium for callus induction from hypocotyl was MS+ZT 0.2 mg/L+6-BA 0.1 mg/L+2,4-D 2.1 mg/L;MS+NAA0.1 mg/L+ZT 0.6 mg/L was the best medium for callus differentiation and adventitious buds differentiation's multiplication;cultivated in the medium 1/2 MS after the adventitious buds disposed 24 h with IAA10 mg/L was the best method for rooting of adventitious buds.The transplanting survival rate of tube seedlings was 94.1% and stable planting survival rate was 98.5% in the greenhouse,the tube seedlings survivaled retained all biological characteristics of the wild Androsace filiformia.