| 欧美杂交杨Pnd-LRR3基因克隆及其抗锈菌侵染表达 |
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| 引用本文： | 陈俏丽,李丹蕾,王峰,邹莉,王志英,牛春阳,王博文,零雅茗,崔嵘,张玉洲.欧美杂交杨Pnd-LRR3基因克隆及其抗锈菌侵染表达[J].东北林业大学学报,2015(3):95-98,103. |
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| 作者姓名： | 陈俏丽 李丹蕾 王峰 邹莉 王志英 牛春阳 王博文 零雅茗 崔嵘 张玉洲 |
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| 作者单位： | 东北林业大学，哈尔滨，150040 |
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| 基金项目： | 中央高校基本科研业务费专项资金项目(DL13BA01);国家“863”计划项目(2013AA102701);黑龙江省博士后基金(LBH-Z11248) |
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| 摘 要： | 为深入探究欧美杂交杨(Populus nigra×P.deltoides)感病的分子机理,以及抗病基因在发病过程中所起的作用,克隆了欧美杂交杨LRR3蛋白编码基因Pnd-LRR3。对Pnd-LRR3基因进行了生物信息学分析,并对其在抗锈菌过程中的功能进行了研究。Q-PCR结果显示,强致病性的落叶松-杨栅锈菌(Melampsora larici-populina)E4菌株接种6 h,Pnd-LRR3基因表达量上调增幅较大,168 h为显著下调。说明Pnd-LRR3基因在E4侵染过程中起到一定抗性作用,但最终无法阻止锈菌的侵染。
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| 关 键 词： | LRR3基因 落叶松-杨栅锈菌 欧美杂交杨 E4株系 Q-PCR |
Genetic Cloning and Gene Expression of Pnd-LRR3 Resistance Gene from Populus nigra×P.deltoids |
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| Chen Qiaoli;Li Danlei;Wang Feng;Zou Li;Wang Zhiying;Niu Chunyang;Wang Bowen;Ling Yaming;Cui Rong;Zhang Yuzhou.Genetic Cloning and Gene Expression of Pnd-LRR3 Resistance Gene from Populus nigra×P.deltoids[J].Journal of Northeast Forestry University,2015(3):95-98,103. |
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| Authors: | Chen Qiaoli;Li Danlei;Wang Feng;Zou Li;Wang Zhiying;Niu Chunyang;Wang Bowen;Ling Yaming;Cui Rong;Zhang Yuzhou |
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| Institution: | Chen Qiaoli;Li Danlei;Wang Feng;Zou Li;Wang Zhiying;Niu Chunyang;Wang Bowen;Ling Yaming;Cui Rong;Zhang Yuzhou;Northeast forest University; |
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| Abstract: | We studied the Pnd-LRR3 gene in the process of rust resistance function , cloned Pnd-LRR3 gene, and analyzed its bioinformatics to explore the molecular mechanism of the hybrid poplar Robusta ( Populus nigra×P.deltoides) and the role of resistance genes in the course of illness .By Q-PCR, the expression quantity of Pnd-LRR3 gene was highly increased when the virulent filial generation of Melampsora larici-populina E4 was inoculated for 6 h, while for 168 h got a significant negative correlation.Pnd-LRR3 gene could play a role in resistance of E4 infection. |
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| Keywords: | LRR3 gene Melampsora larici-populina Populus nigra×P deltoides E4 strains Q-PCR |
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