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轻度盐碱地转betA基因小黑杨的生长表现
引用本文:穆怀志,李志新,李玉珠,唐潇威,姜静,杨传平,刘桂丰.轻度盐碱地转betA基因小黑杨的生长表现[J].东北林业大学学报,2009,37(11).
作者姓名:穆怀志  李志新  李玉珠  唐潇威  姜静  杨传平  刘桂丰
作者单位:1. 林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨,150040
2. 大庆市绿化工程有限责任公司
基金项目:黑龙江省科技攻关重点项目 
摘    要:在前期获得11个转胆碱脱氢酶基因(betA)小黑杨株系的基础上,将转基因株系与非转基因对照株系在轻度盐碱地上造林,5 a后对试验林的转基因株系进行PCR分子检测,并运用SPSS软件对其树高、胸径、材积和保存率4个生长性状进行了方差分析.结果表明:转betA基因小黑杨外源基因稳定,目前尚未丢失;转基因株系与对照株系间树高、胸径、材积和保存率的差异均达到极显著水平;利用隶属函数法选择的转基因株系T1、T6和T8,为生长速度快、耐盐性高的优良株系.

关 键 词:小黑杨  betA基因  生长性状  耐盐性

Growth Manifestation of Transgenic Populus simonii×P. nigra with betA Gene on Low-grade Salinate Fields
Mu Huaizhi,Li Zhixin,Li Yuzhu,Tang Xiaowei,Jiang Jing,Yang Chuanping,Liu Guifeng.Growth Manifestation of Transgenic Populus simonii×P. nigra with betA Gene on Low-grade Salinate Fields[J].Journal of Northeast Forestry University,2009,37(11).
Authors:Mu Huaizhi  Li Zhixin  Li Yuzhu  Tang Xiaowei  Jiang Jing  Yang Chuanping  Liu Guifeng
Institution:Mu Huaizhi,Li Zhixin(Key Laboratory of Forest Tree Genetic Improvement , Biotechnology,Ministry of Education(Northeast Forestry University),Harbin 150040,P.R.China),Li Yuzhu,Tang Xiaowei(Daqing Afforestation Engineering Limited Liability Company),Jiang Jing,Yang Chuanping,Liu Guifeng(Key Laboratory of Forest Tree Genetic Improvement , Biotechnology,Ministry of Education(Northeast Forestry University))
Abstract:An afforestation test of transgenic and untransformed control plants was conducted on low-grade salinate fields by means of the obtained 11 betA transgenic Populus simonii × P. nigra plants in the previous work. Five years later, PCR detection for the transgenic plants was done, and variance analysis for tree height, diameter at breast height, volume, and survival rate was also made with SPSS software. Result showed that betA gene still existed in the genome of the transgenic plants. Significant variations were found in tree height, diameter at breast height, volume, and survival rate between the transgenic and untransformed control plants. Tl, T6 and T8 transgenic lines were identified as fast growing plants with high saline tolerance by subordinate function.
Keywords:Populus simonii ×P  rrigra  betA gene  Growth characters  Saline tolerances
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