| 奥利亚罗非鱼和尼罗罗非鱼核型及DNA含量的比较研究 |
| |
| 引用本文： | 尹洪滨,潘峰.奥利亚罗非鱼和尼罗罗非鱼核型及DNA含量的比较研究[J].东北林业大学学报,1995,23(1):46-51. |
| |
| 作者姓名： | 尹洪滨 潘峰 |
| |
| 作者单位： | 黑龙江水产研究所，中国水产科学研究所淡水渔业研究中心，东北林业大学 |
| |
| 摘 要： | 采用ＰＨＡ体内培养法，取鱼体肾细胞用空气干燥法制备奥利亚罗非鱼和尼罗罗非鱼的染色体，经核型分析，奥利亚罗非鱼为２ｎ＝４４，６ｓｍ＋２４ｓｔ＋１４ｔ；ＮＦ＝５０。尼罗罗非鱼为２ｎ＝４４，６ｓｍ＋２４ｓｔ＋１４ｔ；ＮＦ＝５０。经流式细胞仪测量外周血红血球的ＤＮＡ含量，奥利亚罗非鱼为２Ｃ＝２．２２ｐｇ（２．２２ｐｇ／Ｎｕｃｌ）；尼罗罗非鱼为２Ｃ＝２．２７ｐｇ（２．２７ｐｇ／Ｎｕｃｌ）。经对比分析，两种鱼
|
| 关 键 词： | 罗非鱼 奥利亚罗非鱼 尼罗罗非鱼 染色体 DNA |
STUDIES ON KARYOTYPES AND CELLULAR DNA CONTENT OF SAROTHERODON AUREA AND S.NILOTICA |
| |
| Yin Hongbin,Fan Zhaoting.STUDIES ON KARYOTYPES AND CELLULAR DNA CONTENT OF SAROTHERODON AUREA AND S.NILOTICA[J].Journal of Northeast Forestry University,1995,23(1):46-51. |
| |
| Authors: | Yin Hongbin Fan Zhaoting |
| |
| Institution: | Yin Hongbin;Fan Zhaoting(Heilonajiang Fishery Research Institute)Pan Feng(Freshwater Fishery Research Center of Fishery Product Science and Research institute of China)Sun Zhongwu(Northeast Forestry University) |
| |
| Abstract: | Adopted the PHA intra-cultivation, the kidney cells of Sarotherodon aurea and S.nilotica were taken out to make the metaphase chromosome specimens by air-drying method. The idiograms were established on the basis of their karyotypes. The results indicate that the chromosome number of S. aurea is 2n=44,chromosome formula is 6 st+24 st+14 t, NF= 50, and the chromosome number of S. nilotica is 2n=44, chromosome formula is 6 sm+24 st+14 t, NF= 50. Flow cytometric was used to determine the DNA content of peripheral red blood cells of these two fish. The absolute DNA content determined is that in S. aurea it is 2c=2. 22 pg (2. 22 pg/Nucl) and in S. nilotica 2c=2. 27 pg (2. 27 pg/Nucl). The result of comparison is that the difference between these two species is not apparent in karyotype and DNA content, Which shows that they are quite close to each other in terms of classification status. |
| |
| Keywords: | Sarotherodon aurea Sarotherodon nilotica Chromosome DNA content |
| 本文献已被 CNKI 维普 等数据库收录！ |
