| 胡杨、柽柳总RNA提取方法的建立 |
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| 引用本文： | 王玉成,薄海侠,杨传平.胡杨、柽柳总RNA提取方法的建立[J].东北林业大学学报,2003,31(5):99-100. |
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| 作者姓名： | 王玉成 薄海侠 杨传平 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 国家“973”树木育种的分子基础资助项目 (G1 9990 1 60 0 0 ) |
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| 摘 要： | 以胡杨和柽柳的叶片为材料，建立了一种从植物组织中快速分离总RNA的方法。该方法具有三个显著的特点：RNA提取周期短，应用改进的LiCl沉淀RNA方法，仅需l0min即可；方法简单易行，仅需要几种常用试剂，操作步骤简单；提取的RNA杂质少，得率高，并能在提取过程中较有效抑制RNA的褐化效应。RNA质量可以满足cDNA文库构建、DDRT—PCR等要求。
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| 关 键 词： | 胡杨 柽柳 RNA提取方法 植物组织 提取周期 褐化效应 质量 文库构建 |
A Method for Rapid Isolation of Total RNA from Tamarix and Populus euphratica Oliv |
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| Wang Yucheng,Bo Haixia,Yang Chuanping.A Method for Rapid Isolation of Total RNA from Tamarix and Populus euphratica Oliv[J].Journal of Northeast Forestry University,2003,31(5):99-100. |
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| Authors: | Wang Yucheng Bo Haixia Yang Chuanping |
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| Abstract: | A method of rapid isolation of total RNA was developed by using fresh leaves of Tamarix and Populus euphratica Oliv as test materials. This method has three obvious advantages: the cycle of isolation is short, if using improved method of RNA deposition by LiCl it only need 10 minute to deposit RNA; it is simple and only need some kinds of common reagents; isolated RNA has high purity and browning effect can be inhibited by this method. The qualities of RNA can meet the demands of cDNA library construction and DDRT-PCR reaction. |
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| Keywords: | RNA isolation Plant tissues LiCl deposition |
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