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黄山松群体酶基因遗传变异的研究
引用本文:范义荣,毛迎春,李兰英,戴建良,刘世芳.黄山松群体酶基因遗传变异的研究[J].东北林业大学学报,1999,27(3):14-19.
作者姓名:范义荣  毛迎春  李兰英  戴建良  刘世芳
作者单位:浙江林学院,临安,311300
基金项目:国家自然科学基金,浙江省自然科学基金
摘    要:应用等位酶分析法,分析了18个林分群体(小群体)280多株黄山松种子胚乳的EST和MDH酶的酶基因。结果表明:EST酶受3个基因位点调控,其中2个位占 有等位基因5个、4个,平均为3.58个;MDH酶受4个基因位点调控,其中3个位点各有等位基因3个、5个、5个,平均为2.81个。基因位点均是多态的。等位基因在群体中的分布不同。EST酶基因位点的平均期望杂合度为0.562,MDH酶基因位点的平均期望

关 键 词:黄山松  酶基因  基因频率  遗传距离  类群划分  群体变异

Study on the Genetic Variation of Enzyme Genes in Pinus Taiwanensis Population
Fan Yirong,Mao Yingchun,Li Lanying,Dai Jianliang,Liu Shifang.Study on the Genetic Variation of Enzyme Genes in Pinus Taiwanensis Population[J].Journal of Northeast Forestry University,1999,27(3):14-19.
Authors:Fan Yirong  Mao Yingchun  Li Lanying  Dai Jianliang  Liu Shifang
Abstract:The genetic variation of EST and MDH allozyme genes was studied using isozyme loci analysis of seed endosperms in 18 natural stand population (separate lesser populations) of Pinus taiwanensis from 5 provinces. The results are as follows: EST enzymes are affected by 3 gene loci,two of which have a respective allele of 5 and 4,averaging 3. 58; MDH enzymes are affected by 4 gene loci,3 of which have a respective allele of 3. 5 and 5,averaging 2. 81. Gene loci are polymorphic,and alleies are unevenly distributed in different populations. The average expected heterozy gosity (He) of gene loci in EST enzymes is 0. 562,and that in MDH enzymes is 0. 418,fixation index F is -0. 087,demonstrating a higher proportion of lesser populations composed of heterozygotes;with EST enzyme,genetic distance D is 0. 1729,while with MDH enzymes D is 0. 2934,indicating a 14% of variation and a sharp difference in genetics among lesser populations. 17 populations can be divided into 7 to 8 categories based on D values.
Keywords:Pinus taiwanensis  Enzyme gene  Gene frequency  Genetic distance  Division of population  Population variation
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