筇竹组培快繁技术

以筇竹种胚为外植体,MS为基本培养基,通过无菌体系的建立、丛芽诱导增殖、生根培养研究其组培技术,筛选移栽介质。结果表明:在组培无菌条件下,采用体积分数0.5%甲醛(12 h)→体积分数75%酒精(15 min)→体积分数0.1%升汞(8 min)的处理效果较为理想,筇竹种胚污染率为3.33%,萌芽率为86.21%,成活率为83.33%;筛选出MS+2.0 mg·L^-16-BA+0.2 mg·L^-1NAA适合筇竹种胚的丛芽诱导;1/2MS+0.3 mg·L^-1NAA+1.0 mg·L^-1KT+1.0 mg·L^-16-BA适合筇竹分化丛芽增殖;1/2MS+0.3 mg·L^-16-BA+2.0mg·L^-1NAA+0.1 mg·L^-1IBA适合筇竹的生根培养;m(河沙)∶m(珍珠岩)∶m(腐殖土)=1∶1∶2的基质为最佳移栽质量配比。

Technical Research on Rapid Propagation of Qiongzhuea tumidinoda Tissue Culture

The embryos of Qiongzhuea tumidinoda were used as explants, MS was the basic medium, and the tissue culture technique was studied through the establishment of sterile system, bud-induced proliferation and rooting culture, and the transplanted soil was screened. The results showed that under the aseptic conditions of tissue culture, the treatment with 0.5% formaldehyde(12 h)→75% alcohol(15 min)→0.1% mercuric chloride(8 min) was ideal, and the contamination rate of Q. tumidinoda seed embryo was 3.33%. The survival rate was 86.21%, and the survival rate was 83.33%. MS+6-BA2.0 mg·L^-1+NAA0.2 mg·L^-1 was selected for the induction of shoots of Q. tumidinoda seed embryos;1/2 MS+NAA0.3 mg·L^-1+KT1. 0 mg·L^-1+ 6-BA1.0 mg·L^-1 is suitable for bamboo differentiation bud proliferation;1/2 MS+6-BA0.3 mg·L^-1+NAA2 mg·L^-1+IBA0.1 mg·L^-1 is suitable rooting of Q. tumidinoda;Hesha∶Perlite∶The 1∶1∶2 substrate of humus is the best transplant ratio.