| 咪唑型离子液体溶液中纤维素酶荧光活性测定 |
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| 引用本文： | 刘婷婷,张鑫艺,隋小宇,陈刚,王慧玉,张杰,孙辑凯,徐涛.咪唑型离子液体溶液中纤维素酶荧光活性测定[J].东北林业大学学报,2021,49(4):103-105,120. |
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| 作者姓名： | 刘婷婷 张鑫艺 隋小宇 陈刚 王慧玉 张杰 孙辑凯 徐涛 |
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| 作者单位： | 齐齐哈尔医学院,齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省自然科学基金项目(C2017069)。 |
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| 摘 要： | 为考查纤维素酶在离子液体溶液中的酶活,采用荧光光谱分析法对离子液体中纤维素酶的荧光活性测定,研究离子液体的浓度、类型对纤维素酶的活性影响。结果表明:纤维素酶在咪唑类离子液体溶液中荧光活性随离子液体浓度的升高而降低。离子液体为C4 mim]Cl、C4 mim]Br,且浓度为0.1 mol/L使纤维素酶的荧光强度减低最小,即对酶活性影响最小。
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| 关 键 词： | 纤维素酶 离子液体 荧光分析 酶活 稳定性 |
Determination of Cellulase Fluorescence Activity in Imidazole Ionic Liquid Solution |
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| Liu Tinging,Zhang Xinyi,Sui Xiaoyu,Chen Gang,Wang Huiyu,Zhang Jie,Sun Jikai,Xu Tao.Determination of Cellulase Fluorescence Activity in Imidazole Ionic Liquid Solution[J].Journal of Northeast Forestry University,2021,49(4):103-105,120. |
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| Authors: | Liu Tinging Zhang Xinyi Sui Xiaoyu Chen Gang Wang Huiyu Zhang Jie Sun Jikai Xu Tao |
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| Institution: | (Qiqihar Medical University,Qiqihar 161006,P.R.China) |
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| Abstract: | Using imidazole-based ionic liquids as extraction solvents for medicinal plants, the appropriate amount of cellulase can be added to improve the extraction efficiency of ionic liquid solutions. There is a necessary to examine cellulase enzyme activity in the ionic liquid solution using fluorescence spectrometry. The effects of concentration and type of ionic liquid on the activity of cellulase was studied. The fluorescence activity of cellulase in imidazole ionic liquid solution decreased with the increase of ionic liquid concentration. The ionic liquids of C4 mim]Cl, C4 mim]Br, and 0.1 mol/L had minimize the fluorescence intensity of the cellulase, that is, it has the least effect on the enzyme activity. |
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| Keywords: | Cellulase Ionic liquid Fluorescence analysis Enzyme activity Stability |
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