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天牛基因组DNA的提取方法
引用本文:胡艳红,迟德富,种伟,程红,刘延宾,王雷,胡汇文.天牛基因组DNA的提取方法[J].东北林业大学学报,2006,34(2):38-41.
作者姓名:胡艳红  迟德富  种伟  程红  刘延宾  王雷  胡汇文
作者单位:东北林业大学,哈尔滨,150040
基金项目:国家自然科学基金(39970620),教育部高校青年教师奖(2000年度)资助。
摘    要:通过试验探索出3种可以快速、简便地从天牛干标本、液浸标本和新鲜标本中提取DNA模板的方法。对所获得的基因组扩增分析表明,经过PCR之后的DNA能呈现明显的多态性,同种天牛用同一引物扩增幼虫和成虫所获得的结果相同,可以认为,RAPD技术可以应用于天牛成虫干标本及幼虫浸渍标本的鉴定及系统发育研究中,也证明了这3种方法用于提取不同保存时间、不同种天牛基因组的DNA是切实可行的。

关 键 词:天牛  基因组DNA  提取方法  PCR
收稿时间:2005-04-25
修稿时间:2005-04-25

Extraction Method for Genome DNA from Longicorn Beetles
Hu Yanhong,Chi Defu,Chong Wei,Cheng Hong,Liu Yanbin,Wang Lei,Hu Huiwen.Extraction Method for Genome DNA from Longicorn Beetles[J].Journal of Northeast Forestry University,2006,34(2):38-41.
Authors:Hu Yanhong  Chi Defu  Chong Wei  Cheng Hong  Liu Yanbin  Wang Lei  Hu Huiwen
Institution:Northeast Forestry University, Harbin 1500413, P. R. China
Abstract:An experiment was conducted to explore extraction methods for DNA from dry, dipped and fresh samples of longicorn beetles. Three methods to extract quickly and simply were obtained. Analysis of the obtained genome amplification showed that DNA after PCR presented obvious polymorphism, and the results were the same using the same primer to amplify the larva and imago of the same species. It indicates that RAPD technique can be applied to the identification of dry samples and dipped larva sample of longicorn beetles as well as the research of phylogeny. These three methods for extracting genome DNA from different species of longicorn beetles at different preserved time are feasible.
Keywords:Longicorn beetles  Genome DNA  Extraction methods  PCR
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