| 一种制备无菌松材线虫的方法 |
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| 引用本文： | 朱丽华,季锦衣,吴小芹,王张丽,叶建仁.一种制备无菌松材线虫的方法[J].东北林业大学学报,2011,39(6):65-67,71. |
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| 作者姓名： | 朱丽华 季锦衣 吴小芹 王张丽 叶建仁 |
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| 作者单位： | 南京林业大学; |
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| 基金项目： | 国家重点基础研究发展计划(2009CB119200); 江苏省博士后基金(0802048C); 江苏省有害生物入侵预防与控制重点实验室开放基金项目(PMIS200802) |
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| 摘 要： | 报道了一种通过用H2O2处理松材线虫卵进而获得无菌松材线虫的方法.实验结果表明,15%H2O2处理对松材线虫卵有一定影响,随着处理时间延长,松材线虫孵化率下降,处理30min以上使其孵化率降低至10%以下;15%H2O2处理时间对获得无菌松材线虫有较大影响,其中,处理60min后获得无菌线虫的机率达70%.在无菌条件下...
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| 关 键 词： | 松材线虫 H2O2 无菌 致病性 |
A Method for Obtaining Aseptic Pine Wood Nematode |
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| Zhu Lihua,Ji Jinyi,Wu Xiaoqin,Wang Zhangli,Ye Jianren.A Method for Obtaining Aseptic Pine Wood Nematode[J].Journal of Northeast Forestry University,2011,39(6):65-67,71. |
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| Authors: | Zhu Lihua Ji Jinyi Wu Xiaoqin Wang Zhangli Ye Jianren |
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| Institution: | Zhu Lihua,Ji Jinyi,Wu Xiaoqin,Wang Zhangli,Ye Jianren(College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,P.R.China) |
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| Abstract: | This paper describes a method for obtaining aseptic pine wood nematode(Bursaphelenchus xylophilus) by egg disinfection.Results showed that 15 percent hydrogen peroxide had a profound effect on egg hatching of pine wood nematode.The hatching rate of the pine wood nematode decreased with the prolonged processing time,and it decreased to less than 10 percent by soaking the eggs in 15 percent hydrogen peroxide for more than 30 min.The obtained aseptic pine wood nematode was obviously influenced by egg processin... |
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| Keywords: | Bursaphelenchus xylophilus Hydrogen peroxide Asepticism Pathogenicity |
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