| 不同施肥处理对毛竹根际微生物影响及其PCR-DGGE分析 |
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| 引用本文： | 刘玮,邓光华,张嘉超,杨清培.不同施肥处理对毛竹根际微生物影响及其PCR-DGGE分析[J].东北林业大学学报,2011,39(5):50-53. |
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| 作者姓名： | 刘玮 邓光华 张嘉超 杨清培 |
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| 作者单位： | 江西农业大学;湖南大学; |
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| 基金项目： | 国家“十一五”林业科技支撑计划课题(2006BAD03A0703); 江西省教育厅科学技术研究项目(GJJ08192); 江西农业大学自然科学基金青年项目(2980);江西农业大学自然科学基金自由申请项目(2945) |
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| 摘 要： | 采用PCR-DGGE技术对不同施肥处理毛竹根际微生物多样性特征进行研究.结果表明:采用改进的蛋白酶K-CTAB法提取的毛竹土壤DNA经PCR扩增的产物经DGGE检测后得到的电泳条带清晰且分离效果好,可以明显反映出毛竹根际微生物多样性的变化.根际微生物种群组成特征受施肥量、肥料种类影响很大,施肥能够增加毛竹根际微生物的多...
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| 关 键 词： | 毛竹 根际微生物 PCR-DGGE分析 施肥处理 |
Influence of Fertilizer Application on Rhizosphere Microbial Diversity of Phyllostachys edulis Based on PCR-DGGE Analysis |
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| Liu Wei,Deng Guanghua,Zhang Jiachao,Yang Qingpei.Influence of Fertilizer Application on Rhizosphere Microbial Diversity of Phyllostachys edulis Based on PCR-DGGE Analysis[J].Journal of Northeast Forestry University,2011,39(5):50-53. |
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| Authors: | Liu Wei Deng Guanghua Zhang Jiachao Yang Qingpei |
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| Institution: | Liu Wei,Deng Guanghua(College of Landscape Architecture and Art,Jiangxi Agricultural University,Nanchang 330045,P.R.China),Zhang Jiachao(Hunan University),Yang Qingpei(Jiangxi Agricultural University) |
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| Abstract: | A Polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) method was used to determine the diversity of rhizosphere microorganisms of Phyllostachys edulis after different fertilizer application.Total community DNA was extracted and purified from soils by an improved protease K-CTAB method,and then amplified using eubacterial 16S rDNAs primers.Their PCR products were analyzed by DGGE to obtain bacterial community patterns.The electrophoresis lanes obtained by PCR-DGGE were legible and dis... |
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| Keywords: | Phyllostachys edulis Rhizosphere microorganisms PCR-DGGE analysis Fertilizer application |
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