| 二色补血草LbGST基因的原核表达载体构建与表达 |
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| 引用本文： | 刁桂萍,刘彬令,雷作霖,郭荫久,王玉成.二色补血草LbGST基因的原核表达载体构建与表达[J].东北林业大学学报,2011,39(4):125-126. |
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| 作者姓名： | 刁桂萍 刘彬令 雷作霖 郭荫久 王玉成 |
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| 作者单位： | 东北林业大学; |
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| 基金项目： | 中央高校基本科研业务费专项资金项目(DL09AA02); 东北林业大学大学生创新性实验项目 |
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| 摘 要： | 从二色补血草(Limonium bicolor)中分离出一个编码谷胱甘肽硫转移酶(LbGST)基因,并将该基因构建到pGEX-4T-2原核表达载体,转入大肠杆菌(Escherichia coli)BL21中,利用0.1 mmol/L的IPTG诱导该基因在大肠杆菌BL21中进行表达.SDS-PAGE检测表明,出现了特异的...
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| 关 键 词： | 二色补血草 谷胱甘肽硫转移酶 原核表达 |
Cloning and Prokaryotic Expression of a Glutathione S-Transferase Gene from Limonium bicolor |
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| Diao Guiping,Liu Binling,Lei Zuolin,Guo Yinjiu,Wang Yucheng.Cloning and Prokaryotic Expression of a Glutathione S-Transferase Gene from Limonium bicolor[J].Journal of Northeast Forestry University,2011,39(4):125-126. |
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| Authors: | Diao Guiping Liu Binling Lei Zuolin Guo Yinjiu Wang Yucheng |
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| Institution: | Diao Guiping,Liu Binling,Lei Zuolin,Guo Yinjiu,Wang Yucheng(School of Forestry,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | A glutathione S-transferase(GST) gene was cloned from a cDNA library of Limonium bicolor(Bunge) Kuntze(Plumbaginaceae).The LbGST gene was inserted into the prokaryotic expression vector pGEX-4T-2,and then transformed into E.coli BL21.The expression of GST gene in E.coli BL21 was induced by 0.01 mmol/L IPTG.SDS-PAGE results indicated that a specific band with molecular weight of 50 kDa displayed by transformants was the target peptide,indicating that it was successfully expressed in E.coli.This research will... |
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| Keywords: | Limonium bicolor Glutathione S-transferase Prokaryotic expression |
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