基于SSR标记的楸树遗传多样性及核心种质构建

利用SSR标记对192个楸树种质资源进行遗传多样性和亲缘关系研究。试验筛选出13对引物对192份供试材料进行扩增,共获得89个等位基因位点,有效等位基因平均为3.795 9,Shannon’s多样性指数平均值为0.506 6;Nei’s遗传多样性平均值为0.667 7。用MEGA6.0软件对192份楸树材料进行遗传距离分析,通过聚类分析构建出供试材料楸树种质资源间的聚类图。利用SSR分子标记,采用多次聚类结合位点优先的取样策略,比较了样本数不同的4个核心样本群的等位基因数、有效等位基因数、Shannon’s指数和Nei’s遗传多样等参数,初步构建了192份楸树种质材料的46份核心种质。核心种质保留了初始种质23.96%的样品。

Genetic Diversity Analysis and Primary Core Collection of Catalpa bungei Germplasm with SSR Markers

Constructing core collection is a useful way to improve efficiency of conservc and manage species germplasm.We used SSR markers for the genetic diversity analysis and genetic relationship research with 192 Catalpa bungei germplasm,selected 13 pairs of SSR primers,amplified all of the 192 samples by these 13 pairs of primers,and found 89 alleles.The average effective number of allele (Ne) was 3.795 9,average of Shannon's diversity index (Ⅰ) was 0.506 6,and Nei's genetic diversity (H) average number was 0.667 7.The analysis of genetic distance of 192 C.bungei samples with MEGA6.0,and the construction of the dendrogram of Catalpa samples by cluster analysis was conducted.With SSR markers and the sampling strategy of multiple cluster binding loci preferential,we primarily constructed 4 core sample groups with different numbers.In these 4 groups,46 core collection of the total 192 collected Catalpa trees were compared by the parameters including the number of allele,effective number of allele,Shannon's index and Nei's genetic diversity.The core collections retained 23％ of the original collections.