| CotA漆酶的固定化及其对溴麝香草酚蓝脱色的效果 |
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| 引用本文： | 邢丹,马铭鸿,吴航伸,孙海琼,王珏玉,汪春蕾,赵敏.CotA漆酶的固定化及其对溴麝香草酚蓝脱色的效果[J].东北林业大学学报,2017,45(10). |
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| 作者姓名： | 邢丹 马铭鸿 吴航伸 孙海琼 王珏玉 汪春蕾 赵敏 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 国家林业局"948"项目，国家自然科学基金项目，黑龙江省博士后资助经费 |
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| 摘 要： | 将林地土壤中分离的枯草芽孢杆菌中克隆的CotA漆酶基因,在毕赤酵母菌中进行表达;以海藻酸钙为载体,采用包埋法固定毕赤酵母菌表达的CotA漆酶,固定化效率为32.2%,正交试验优化后的固定化效率比优化前提高了30.1%。优化了游离CotA漆酶及固定化CotA漆酶对溴麝香草酚蓝的脱色条件,在30min内,固定化CotA漆酶在70℃、pH为8.0时,游离CotA漆酶在60℃、pH为7.6时,对溴麝香草酚蓝溶液的脱色率最高,分别达到76.81%和67.12%,说明固定化CotA漆酶比游离CotA漆酶在较高温度和碱性条件下活性更高。
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| 关 键 词： | CotA漆酶 溴麝香草酚蓝 染料脱色 |
Immobilization of CotA Laccase and Decoloring Bromothymol Blue |
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| Xing Dan,Ma Minghong,Wu Hangshen,Sun Haiqiong,Wang Jueyu,Wang Chunlei,Zhao Min.Immobilization of CotA Laccase and Decoloring Bromothymol Blue[J].Journal of Northeast Forestry University,2017,45(10). |
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| Authors: | Xing Dan Ma Minghong Wu Hangshen Sun Haiqiong Wang Jueyu Wang Chunlei Zhao Min |
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| Abstract: | CotA laccase gene from Bacillus subtilis isolated from forest soil was expressed in Pichia pastoris .The immobilization efficiency of CotA laccase expressed fromP ichia pastoir s was 32.2%with calcium alginate as carrier .The immobilization efficiency was increased by 30.1%through optimizing immobilization condition under orthogonal experiment .The highest decolorization rate of bromothymol blue by immobilized CotA laccase was 76.81%under 30 min, 70℃and pH of 8.0.The highest decolorization rate of bromothymol blue by free laccase was 67.12%under 30 min, 60℃and pH of 7.6.The results indicated that immobilized CotA laccase had higher enzyme activity than free CotA laccase under heat and alkali condition . |
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| Keywords: | CotA laccase Bromothymol blue Dyes decolorizing |
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