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| 狼亚种分化的RAPD分子证据 | |
| 引用本文： | 王秀辉,高中信,于学伟,崔岩.狼亚种分化的RAPD分子证据[J].东北林业大学学报,2000,28(5):110-113. |
| 作者姓名： | 王秀辉 高中信 于学伟 崔岩 |
| 作者单位： | 1. 河北省林业厅林政资源处,石家庄,050051 2. 东北林业大学 3. 黑龙江省防疫站病毒所 |
| 摘 要： | 对来自我国东北、内蒙、甘肃、新疆等１０个地区的２１只狼（Ｃａｎｉｓ ｌｕｐｕｓ）应用ＲＡＰＤ方法进行亚种分化研究。筛选出２５个随机引物。通过对ＲＡＰＤ产生的谱带进行比较。找出共享片段，计算共享度和遗传距离。并进行聚类分析，做出聚类图，确定亲缘关系和分类地位。研究结果表明：（１）中国南北方狼有分化。在ＤＮＡ序列上存在着明显差异；（２）把所研究地区的狼划分为５个亚种，即：云贵群体（云南群体和贵州群体）和东南群体（福建群体、浙江群体和广东群体）亚种；东北群体、河北群体亚种；甘肃群体亚种；内蒙群体亚种；新疆群体亚种。 |
| 关 键 词： | RAPD 分子系统树 亚种分类 狼 DNA序列 |
Molecular Evidence of RAPD in Wolf Subspecies Differentiation |
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| Wang Xiuhui,Gao Zhongxin,Yu Xuewei,Cui Yan.Molecular Evidence of RAPD in Wolf Subspecies Differentiation[J].Journal of Northeast Forestry University,2000,28(5):110-113. | |
| Authors: | Wang Xiuhui Gao Zhongxin Yu Xuewei Cui Yan |
| Institution: | Wang Xiuhui (Division of Forestry Administration and Resource, Hebei Forestry Department, Shijiazhuang 050051, P. R. China)Gao Zhongxin ,Yu Xuewei (Northeast Forestry University)Cui Yan (Virus Institute of Anti-epidemic Clinic, Heilongjiang Province) |
| Abstract: | solves (Canis lupus). which came from 10 areas in China, Northeast area, Inner Mongolia area. Gansu area and Xinjiang area, were studied using random amplified polymorphic DNA(RAPD) markers. 25 primers were screened and RAPD bands were obtained. the shared fragments and genetic distances were calculated by studying the electrophoresis patterns of RAPD. The results showed that the DNA orders of wolves in tile south and north of China exist apparently differences. The wolves in the research areas were divided into 5 subspecies. Yungui and Southeast populations, Northeast and Hebei populations, Gansu pupulation. Inner. mongolia population and Xinjiang populations. Yungui population includes Yunan and Guizhou populations. and Southeast population includes Fujian. Zhejiang and Guangdong populations. |
| Keywords: | DNA RAPD Phylogenetic tree: Subspecies taxonomy: Polymorphism |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |