| 冰冻切片技术在高山红景天细胞学研究中的应用 |
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| 引用本文： | 刘剑锋,阎秀峰,程云清,李霞.冰冻切片技术在高山红景天细胞学研究中的应用[J].东北林业大学学报,2006,34(4):110-110,119. |
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| 作者姓名： | 刘剑锋 阎秀峰 程云清 李霞 |
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| 作者单位： | 1. 东北林业大学,哈尔滨,150040
2. 吉林师范大学 |
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| 基金项目： | 黑龙江省博士后科研启动基金 |
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| 摘 要： | 为了解决利用普通石蜡切片观察植物样品需要进行脱水、浸透、包埋操作且耗时长的问题,以高山红景天为试材,对其根和茎进行冰冻切片,过程简单有效且图版清晰,在1d内即可获得高质量图片。冰冻切片的关键步骤是选择合适的甘油浓度、切片时采用的温度及展片技术。
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| 关 键 词： | 冰冻切片 高山红景天 细胞学 |
| 收稿时间： | 2005-07-06 |
| 修稿时间： | 2005-07-06 |
Application of Cryo-sectioning Technique to Cytology Studies of Rhodiola sachalinensis A. Bor |
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| Liu Jianfeng,Yan Xiufeng,Cheng Yunqing,Li Xia.Application of Cryo-sectioning Technique to Cytology Studies of Rhodiola sachalinensis A. Bor[J].Journal of Northeast Forestry University,2006,34(4):110-110,119. |
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| Authors: | Liu Jianfeng Yan Xiufeng Cheng Yunqing Li Xia |
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| Institution: | 1 Northeast Forestry University, Harbin 150040 P. R. China; 2 Jilin Normal University |
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| Abstract: | It needs dehydrating, infiltrating and embedding before observing when observing plant specimens with paraffin sections. Aiming to solve this time consuming process, cryo-sectioning technique was used to observe stem and root structure of two-year-old Rhodiola sachalinensis A. Bor. The operating process is simple and efficient, and clear plates can be acquired within one day. The key step is to select a proper concentration of glycerol, a suitable temperature of freezing and an appropriate way of section-flatting. |
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| Keywords: | Cryo-sectioning Rhodiola sachalinensis A Bor Cytology |
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