四川牡丹根际微生物及种子内生菌组成

利用Illumina高通量测序技术研究了马尔康、丹巴、小金、理县4个种源地四川牡丹根际土壤微生物和种子内生菌的组成。测序结果显示:在门水平上共存在19种细菌和6种真菌,其中Proteobacteria(变形菌门)、Nitrospirae(消化螺旋菌门)、Fimicutes(厚壁菌门)和Ascomycota(子囊菌门)在根际土壤和种子中均占优势(相对丰度>10%);在属水平上,共鉴定出181种细菌和92种真菌,其中Leptospirillum(钩端螺菌属)和Penicillium(青霉属)均占优势(相对丰度>10%)。物种多样性(Alpha)分析结果表明:根际微生物的物种丰富度(Chao1)显著高于种子内生菌,而群落多样性(Shannon、Simpson)并无显著差异。群落间组成差异(Beta多样性)分析结果表明:根际微生物和种子内生菌的群落组成差异显著,主要差异为Chloroflexi(绿弯菌门)和Mucoromycota(毛霉菌门)在种子中的显著富集。OUT重叠序列结果显示:同一种源地根际土壤和种子中有70%以上的相同细菌序列,而相同的真菌序列最高约50%,最低约15.52%。与土壤基本理化性质的相关性分析表明:土壤有机碳、氮、磷总质量分数及氮磷有效态质量分数等土壤基本理化性质均会对根际土壤微生物及内生菌的组成结构产生一定影响。

Microbial Composition Associated with the Rhizosphere and Seed Endosphere of Paeonia szechuanica

Illumina platform was used to characterize the microbial community diversity and composition in the rhizosphere and seed endosphere of Paeonia szechuanica. A total of 19 bacterial phyla and 6 fungi phyla were identified, of which Proteobacteria, Nitrospirae, Fimicutes and Ascomycota dominated both the rhizosphere microbe and seeds endophyte(relative abundance>10%). At the genus level, a total of 181 bacteria and 92 fungi were identified, of which Leptospirillum and Penicillium dominated both the rhizosphere microorganism and seed endophytes. Alpha-diversity indicated that the Chao1 index of endophytes was lower than that of rhizosphere microorganisms, but there was no significant difference in the Shannon, Simpson and PD. Beta-diversity indicated the significantly different composition between the rhizosphere microorganisms and seed endophytes, mainly because the relative abundance of Chloroflexi and Mucoromycota in seeds was significantly higher than that in the rhizosphere. In addition, the same in situ rhizosphere bacteria and seed endophytic bacteria have more than 70% of the same sequence, while the same sequence in fungi highest and lowest were 50% and 15.52%, respectively. In addition, the soil basic physical and chemical properties including the contents of organic carbon, nitrogen and phosphorus and their available state have certain effects on rhizosphere and seed endosphere microorganisms.