| 二色补血草硫氧还蛋白过氧化物酶基因LbTPx的克隆及其表达 |
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| 引用本文： | 刁桂萍,杨传平,王玉成.二色补血草硫氧还蛋白过氧化物酶基因LbTPx的克隆及其表达[J].东北林业大学学报,2011,39(8):85-87,115. |
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| 作者姓名： | 刁桂萍 杨传平 王玉成 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 中央高校基本科研业务费专项资金项目 |
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| 摘 要： | 从二色补血草(Limonium bicolor)cDNA文库中分离出一个新的硫氧还蛋白过氧化物酶基因(LbTPx)全长cDNA序列.基因全长1016 bp,其中:5'非翻译区146 bp,3'非翻译区69 bp,开放读码框(ORF)801 bp,共编 码266个氨基酸；编码蛋白的分子质量为47.99× 10-24...
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| 关 键 词： | 二色补血草 硫氧还蛋白过氧化物酶 实时定量PCR |
Cloning and Expression of a Thioredoxin Peroxidase Gene LbTPx from Limonium bicolor |
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| Diao Guiping,Yang Chuanping,Wang Yucheng.Cloning and Expression of a Thioredoxin Peroxidase Gene LbTPx from Limonium bicolor[J].Journal of Northeast Forestry University,2011,39(8):85-87,115. |
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| Authors: | Diao Guiping Yang Chuanping Wang Yucheng |
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| Institution: | (School of Forestry,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | The full length cDNA of a novel Thioredoxin Peroxidase(LbTPx) gene was cloned from a cDNA library of Limonium bicolor.The LbTPx gene cloned is 1016bp in length,which includes a 146 bp of 5’ untranslated region(UTR) and a 69 bp of 3’ untranslated region.This gene has an open reading frame(ORF) of 801bp in length,encoding a protein of 266 amino acid residues with the molecular mass of 47.99×10-24 kg and theoretical pI of 8.83.The expression of LbTPx gene in L.bicolor in response to NaCl,ABA,CuSO4,ZnCl2 and CdCl2 was further investigated using real time quantitative PCR.In both leaf and root of L.bicolor,the expression of LbTPx was induced by NaCl and CuSO4.However,the expression of LbTPx was induced in leaf and inhibited in root of L.bicolor under ABA,ZnCl2 and CdCl2 stresses. |
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| Keywords: | Limonium bicolor Thioredoxin peroxidase Real-time quantitative PCR |
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