Bt对分月扇舟蛾体毒性及体内保护酶系影响

以分月扇舟蛾5龄幼虫为研究对象,采用叶片药膜法测定了Bt对其毒性,研究了致死剂量的Bt对其体内SOD、CAT、POD保护酶活性的影响.结果表明:Bt对分月扇舟蛾36、48、72h的LC30和LC50分别为:24.674、8.578、5.622 ng·L-1和78.311、17.252、11.741 ng· L-1.9 ...

Effect of Bacillus thuringiensis on Activities of Protective Enzymes in Clostera anastomosis

A test was conducted to study the toxic effect of Bacillus thuringiensis(Bt) on the 5th instar larvae of Clostera anastomosis and the effect of lethal dose of Bt on the activities of protective enzymes in C.anastomosis by leaf membrane.Results showed that LC30 and LC50 of Bt against C.anastomosis at 36h,48h,72h were 24.674,8.578,5.622ng·L-1,and 78.311,17.252,11.741ng·L-1 respectively.The activities of SOD,CAT and POD in the larvae treated with 9ng·L-1 of Bt were activated to varying degrees.After treated with 17ng·L-1 of Bt,SOD activity of the larvae increased first and then decreased,and SOD activity reached its maximum at 48h,which was 0.704 times higher than that of the control;CAT activity was slightly higher than that of the control,and a significant difference was only observed at 24h(P<0.01);POD activity showed a rising trend and significant differences were found between 48h and 72h(P<0.01).Different concentrations of Bt destroyed the balance in activities of protective enzymes in the larvae of C.anastomosis,and high levels of free radicals resulted in an irreversible toxic effect on the larvae.