| 婆婆纳组织培养再生体系的建立 |
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| 引用本文： | 张彦妮,李博,安勇,李谷军.婆婆纳组织培养再生体系的建立[J].东北林业大学学报,2010,38(12). |
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| 作者姓名： | 张彦妮 李博 安勇 李谷军 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 黑龙江省博士后科研启动基金; 中央高校基本科研业务费专项资金项目(DL09BA37)资助 |
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| 摘 要： | 以婆婆纳(Veronica didymaTenore)的叶片、茎节、茎段、顶芽为外植体,接种在附加2,4-D、6-BA、NAA等不同质量浓度和组合的培养基中进行离体培养,并比较了不同培养基上不同外植体的诱导率。结果表明,诱导愈伤组织的最佳外植体是叶片,诱导愈伤组织的最佳培养基为MS+2,4-D0.01 mg/L+6-BA1.0 mg/L+NAA1.0 mg/L,愈伤组织诱导率为100%。诱导愈伤组织分化的最适培养基为MS+2,4-D0.01 mg/L+6-BA0.75mg/L+NAA1.75 mg/L,分化率为90%。诱导生根的培养基为l/2MS+NAA0.2 mg/L,生根率为90%。
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| 关 键 词： | 婆婆纳 愈伤组织 组织培养 |
Establishment of Regeneration System for Veronica didyma |
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| Zhang Yanni,Li Bo,An Yong,Li Gujun.Establishment of Regeneration System for Veronica didyma[J].Journal of Northeast Forestry University,2010,38(12). |
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| Authors: | Zhang Yanni Li Bo An Yong Li Gujun |
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| Institution: | Zhang Yanni,Li Bo,An Yong,Li Gujun(School of L,scape Architecture,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | Leaves,stem internodes,stem segments without nodes and apical bud of Veronica didyma,which were used as the explants,were inoculated into the MS medium supplemented with different concentrations of 2,4-D,6-BA,NAA or different hormone combinations.The induction rates of different explants were compared.Result showed that the best explants for callus induction were leaves of V.didyma and the optimum culture medium was MS medium containing 0.01 mgL 2,4-D,1.0 mgL 6-BA and 1.0 mgL NAA,with an induction rate of 1... |
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| Keywords: | Veronica didyma Calli Tissue culture |
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