| 白桦长纤维性状ISSR和SCAR标记的分析 |
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| 引用本文： | 夏德安,魏志刚,杨传平,刘关君.白桦长纤维性状ISSR和SCAR标记的分析[J].东北林业大学学报,2008,36(9). |
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| 作者姓名： | 夏德安 魏志刚 杨传平 刘关君 |
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| 作者单位： | 林木遗传育种与生物技术教育部重点实验室(东北林业大学),哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金，黑龙江省科技攻关项目 |
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| 摘 要： | 通过对天然白桦群体583株个体的纤维长度进行测定,选取其中有代表性的100株个体,利用简单重复序列间区标记技术(Inter-simple sequence repeat,ISSR)进行基因组差异分析,通过扩增条带与性状表现间的多元回归分析,筛选出与白桦纤维长度显著相关的分子标记。经过20个ISSR引物筛选,有4个引物的4条片段与纤维长度呈极显著相关,其中片段"BFLI-3"与纤维长度的回归系数为-0.331,显著性达到1%水平。对此片段进行克隆、测序后,成功转化成与长纤维性状相关的序列特征化扩增区域(Sequence characterized amplified region,SCAR)标记,此标记对短纤维白桦的鉴定效率达76%。
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| 关 键 词： | 白桦 长纤维性状 ISSR SCAR |
Analysis of ISSR and SCAR Markers Associated with Birch Fiber Length Trait |
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| Xia Dean,Wei Zhigang,Yang Chuanping,Liu Guanjun.Analysis of ISSR and SCAR Markers Associated with Birch Fiber Length Trait[J].Journal of Northeast Forestry University,2008,36(9). |
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| Authors: | Xia Dean Wei Zhigang Yang Chuanping Liu Guanjun |
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| Abstract: | After measuring fiber length trait (FLT) of 583 individuals from nature birch population, 100 individuals were selected as representative variety for correlated fragment screening with Inter-simple sequence repeat (ISSR) technique. Four fragments from four primers were identified having significant correlation with FLT based on multiple regression analysis. The correlation coefficient between BFLI-3 and FLT was -0.331, showing a significant difference at the 0.01 level. BFLI-3 was cloned, sequenced and transformed into Sequence Characterized Amplified Region (SCAR) marker. The identifying percentage of short-fiber birch by this SCAR marker reached 76%. |
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| Keywords: | Betula platyphylla Fiber length trait Inter-simple sequence repeat Sequence characterized amplified region |
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