扎龙自然保护区丹顶鹤3个小群体遗传变异水平的比较

应用6个微卫星分子标记位点评价了扎龙自然保护区包括野生、散养和笼养丹顶鹤3个小群体的遗传变异水平,以及野生与散养小群体间的基因流水平。结果表明,3个丹顶鹤小群体均表现出较高且相近的遗传变异水平,杂合度期望值(HE)为野生0.83300,散养0.80000,圈养0.81000,F-Statistics结果有97.5%以上的遗传多样性存在于群体内。同时还应用两种计算方法计算了野生与散养丹顶鹤小群体间基因流水平:应用稀有等位基因法计算两个小群体间的基因流值为每代1.9个个体;应用FST指数计算方法,并根据基因流值计算公式得出基因流值Nm为1个个体。这个基因流水平和机制能够维持扎龙自然保护区野生与散养丹顶鹤小群体间相近且较高的遗传变异水平。

Genetic Variation Level among Three Populations of the Red-crowned Crane in Zhalong National Nature Reserve

Six microsatellite loci were used to assess the genetic variation levels between populations in Zhalong Nature Reserve and levels of gene flow between wild and non-migratory subpopulations of red-crowned crane.Results show that wild,non-migratory and captive populations of red-crowned crane have high genetic variation levels.HE value is 0.833 00 for wild population,0.800 00 for non-migratory population and 0.810 00 for captive population.FST results manifest about 97.5% genetic diversity within population and 2.5% genetic diversity between subpopulations.Levels of gene flow shows 1.9 individuals every generation by private allele method and 1 individual every generation by FST method,and the values are enough to maitain a higher genetic variation level between wild and non-migratory populations in Zhalong Nature Reserve.