| 紫丁香、糖槭总RNA的快速提取方法 |
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| 引用本文： | 王玉成,杨传平,姜静.紫丁香、糖槭总RNA的快速提取方法[J].东北林业大学学报,2001,29(6):90-91. |
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| 作者姓名： | 王玉成 杨传平 姜静 |
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| 作者单位： | 东北林业大学, |
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| 基金项目： | 国家“973”耐盐基因克隆资助项目 |
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| 摘 要： | 以酸酚提取RNA的基本原理为基础 ,应用硼砂·CTAB·酸酚法和SDS·酸酚法两种方法成功地提取了紫丁香、糖槭的总RNA ,与常用的异硫氰酸胍方法比较 ,这两种方法具有快速、有效等优越性
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| 关 键 词： | 紫丁香 糖槭 RNA 酸苯酚提取 |
| 修稿时间： | 2001年7月11日 |
Method of Extracting RNA Quickly from Syringa oblata and Acer negundo |
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| Wang Yucheng,Yang Chuanping,Jiang Jing.Method of Extracting RNA Quickly from Syringa oblata and Acer negundo[J].Journal of Northeast Forestry University,2001,29(6):90-91. |
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| Authors: | Wang Yucheng Yang Chuanping Jiang Jing |
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| Abstract: | Based on the acidic phenolic method of extracting RNA, the authors succeed in extracting the total RNA of Syringa oblata and Acer negundo in different ways by means of borax-CTAB- acidic phenolic method and SDS- acidic phenolic method. The two methods have the obvious advantages of celerity and efficiency in extracting RNA from ligneous plants compared with the guanidinium isothiocyanate method. |
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| Keywords: | Syringa oblata Acer negundo RNA Acidic phenolic extraction |
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