邻羟基苯甲酸胁迫下杉木叶片游离氨基酸的变化特征

在杉木自毒物质邻羟基苯甲酸胁迫下,对杉木组培苗叶片游离氨基酸质量分数变化及化感效应进行研究。结果表明:随着胁迫时间的延长、胁迫强度的增大,杉木叶片总游离氨基酸的含量逐渐提高,促进效应显著,胁迫质量浓度与氨基酸总量间呈线性关系;在不同胁迫时间及不同胁迫质量浓度下,天冬氨酸含量最高,其次为谷氨酸,化感效应趋势随胁迫时间的延长和胁迫质量浓度增大,表现为促进作用增强;脯氨酸积累量随胁迫时间的延长和胁迫质量浓度增大而逐渐增加,化感效应由抑制转为促进效应;其他氨基酸对胁迫的反应呈现波动性。

Variation Characteristics of Free Amino Acids in Chinese Fir Leaves under Salicylic Acid Stress

The change of contents of free amino acids in Chinese fir leaves of tissue culture seedlings and the allelopathic effect were studied under salicylic acid stress.Result showed that the contents of free amino acids increased gradually,and the promotion effects of salicylic acid strengthened with stress time prolonging and intensity strengthening.There was a linear relationship between stress concentration and total amino acid.The content of Asp was the highest under different stress periods and different stress concentrations,and then followed by Glu,and the allelopathic effects,presented as promotion effects,tended to become stronger with time prolonging and intensity strengthening.Pro content increased and its allelopathic effect turned from inhibition into promotion with time prolonging and intensity strengthening.Other amino acids showed fluctuation changes with response to salicylic acid stress.