| RP-HPLC法测定细叶杜香叶中4种活性成分的质量分数 |
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| 引用本文： | 于景华,赵丹,付玉杰,孔羽,原树生,祖元刚.RP-HPLC法测定细叶杜香叶中4种活性成分的质量分数[J].东北林业大学学报,2011,39(3):106-107,119. |
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| 作者姓名： | 于景华 赵丹 付玉杰 孔羽 原树生 祖元刚 |
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| 作者单位： | 森林植物生态学教育部重点实验室(东北林业大学),哈尔滨,150040 |
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| 基金项目： | 科技部科技基础性工作专项2007年重点项目，黑龙江省科技攻关项目 |
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| 摘 要： | 为了建立测定细叶杜香叶中秦皮乙素、秦皮素、金丝桃苷和槲皮素质量分数的方法,采用反相高效液相色谱法(色谱条件:色谱柱为Inertsil ODS-SP(5 μm,4.6mm×250 mm,柱号:9K192609)、流动相为V甲醇)∶V(0.2%醋酸)=43∶57、流速1.0 mL·min-1、检测波长350 nm),测得秦...
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| 关 键 词： | 反相高效液相色谱法 细叶杜香 秦皮乙素 秦皮素 金丝桃苷 槲皮素 |
Content Determination of Four Components in Ledum palustre Leaves by RP-HPLC |
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| Yu Jinghua,Zhao Dan,Fu Yujie,Kong Yu,Yuan Shusheng,Zu Yuangang.Content Determination of Four Components in Ledum palustre Leaves by RP-HPLC[J].Journal of Northeast Forestry University,2011,39(3):106-107,119. |
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| Authors: | Yu Jinghua Zhao Dan Fu Yujie Kong Yu Yuan Shusheng Zu Yuangang |
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| Institution: | Yu Jinghua,Zhao Dan,Fu Yujie,Kong Yu,Yuan Shusheng,Zu Yuangang(Key Laboratory of Forest Plant Ecology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China) |
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| Abstract: | An experiment was conducted to establish a RP-HPLC method for content determination of aesculetin,fraxetin,hyperoside and quercetin in Ledum palustre leaves.The reversed-phase high performance liquid chromatography(RP-HPLC) system consisted of an Inertsil ODS-SP column(5μm,4.6mm×250mm,series number: 9KI92609).Volume ratio of methanol to 0.2% acetic acid(43∶57) was used as mobile phase.Flow rate was 1.0mL·min-1,and the detector wavelength was set at 350nm.The average recoveries of aesculetin,fraxetin,hyperoside and quercetin were 99.71%,98.62%,100.13%,and 98.33% with relative standard deviations of 1.45%,1.53%,1.04% and 1.69%,respectively.The RP-HPLC method is simple,accurate and repeatable,and it could be applied to the content determination of four components in L.palustre leaves. |
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| Keywords: | Reversed-phase high performance liquid chromatography Ledum palustre Aesculetin Fraxetin Hyperoside Quercetin |
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