| 周口地区大叶黄杨白粉病菌的鉴定和进化分析 |
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| 引用本文： | 张怡,刘严,康静敏,马晓萌,傅露露,乔月娥,李成伟.周口地区大叶黄杨白粉病菌的鉴定和进化分析[J].东北林业大学学报,2011,39(11):61-63. |
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| 作者姓名： | 张怡 刘严 康静敏 马晓萌 傅露露 乔月娥 李成伟 |
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| 作者单位： | 周口师范学院,周口,466001 |
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| 摘 要： | 对周口地区的大叶黄杨白粉病菌进行了显微形态、核糖体DNA转录间隔区(ITS)及进化树分析。结果表明:在扫描电子显微镜下病菌分生孢子呈圆柱形或椭圆形,无支链;ITS序列与来自日本和阿根廷的Erysipheeuonymi-japonici的ITS序列聚在一个进化枝上。表明来自河南周口地区的白粉病属于E.euonymi-japonici,与日本和阿根廷的E.euonymi-japonici亲缘关系最近。
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| 关 键 词： | 大叶黄杨白粉病 显微形态 分子鉴定 ITS序列 亲缘关系 |
Identification and Phylogenic Analysis of Powdery Mildew on Euonymus japonicus Caused by Erysiphe euonymi-japonici in Zhoukou |
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| Zhang Yi,Liu Yan,Kang Jingmin,Ma Xiaomeng,Fu Lulu,Qiao Yue'e,Li Chengwei.Identification and Phylogenic Analysis of Powdery Mildew on Euonymus japonicus Caused by Erysiphe euonymi-japonici in Zhoukou[J].Journal of Northeast Forestry University,2011,39(11):61-63. |
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| Authors: | Zhang Yi Liu Yan Kang Jingmin Ma Xiaomeng Fu Lulu Qiao Yue'e Li Chengwei |
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| Institution: | (Key Laboratory of Plant Genetics and Molecular Breeeding,Zhoukou Normal University,Zhoukou 466001,P.R.China) |
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| Abstract: | Powdery mildew appearing in colonies of white patches prevalently on leaves of Euonymus japonicus was found in Zhoukou of Henan Province in 2010.The microscopic morphology and molecular identification of the pathogens that cause this disease were studied in terms of internal transcribed spacer(ITS) sequence and phylogenetic trees.The conidia which were borne singly,cylindrical,or ellipsoid-cylindrical without branch were observed under the scanning electron microscopy(SEM).The ITS sequence is highly homologous to two ITS sequences from Erysiphe euonymi-japonici isolates from Japan and Argentina,while the phylogenetic tree demonstrated that they were clustered into one clade.It suggested that the powdery mildew fungus on E.japonicus in Zhoukou belongs to E.euonymi-japonici,and is close to the isolates from Japan and Argentina according to genetic relationship. |
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| Keywords: | Erysiphe euonymi-japonici Microscopic morphology Molecular identification ITS sequences Genetic relationships |
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