| 白桦木材蓝变生防菌(Bacillussubtilis)B26液体发酵条件的优化 |
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| 引用本文： | 韩丽,常建民,张柏林,孙薇,王雨.白桦木材蓝变生防菌(Bacillussubtilis)B26液体发酵条件的优化[J].东北林业大学学报,2011,39(12):71-73. |
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| 作者姓名： | 韩丽 常建民 张柏林 孙薇 王雨 |
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| 作者单位： | 北京林业大学 北京 100083 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 采用单因素试验法和正交试验法对生防菌株B26液体发酵条件进行了优化,得到最适发酵培养基配方为:蔗糖1.5％、蛋白胨1.5％、CaCl2 0.15％、MgSO4·7H2O 0.20％；采用单因素试验法对其培养条件进行优化,确定最适培养条件为:温度35℃、初始pH值7.5、锥形瓶体积为250 mL,发酵液为120 mL,接...
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| 关 键 词： | 白桦 木材蓝变 枯草芽孢杆菌 发酵 |
Optimization of Fermentation Conditions of Bacillus subtilis B26 Against Sapstain Fungi on Betula platyphylla |
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| Han Li,Chang Jianmin,Zhang Bolin,Sun Wei,Wang Yu.Optimization of Fermentation Conditions of Bacillus subtilis B26 Against Sapstain Fungi on Betula platyphylla[J].Journal of Northeast Forestry University,2011,39(12):71-73. |
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| Authors: | Han Li Chang Jianmin Zhang Bolin Sun Wei Wang Yu |
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| Institution: | (College of Material Science and Technology,Beijing Forestry University,Beijing 100083,P.R.China) |
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| Abstract: | The fermentation culture medium for the strain B26 was studied by single factor experiments and orthogonal experiments.The optimal culture medium formula was determined as sucrose 1.5%,peptone 1.5%,CaCl2 0.15%,and MgSO4·7H2O 0.20%.The fermentation conditions were obtained by single factor experiments as temperature 35 degrees C,initial pH of the medium 7.5,fermentation fluid 120 mL in a 250 mL conical flask,inoculation volume 8%,rotation speed 130 r/min,and fermentation time 72 h.The inhibition zone diameter of the fermentation liquid reached 19.00 mm under this optimized conditions. |
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| Keywords: | Betula platyphylla Sapstain Bacillus subtilis Fermentation |
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