| 产几丁质酶酵母菌的筛选与鉴定 |
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| 引用本文： | 惠丰立,冯金荣,杨柯金,文祯中.产几丁质酶酵母菌的筛选与鉴定[J].东北林业大学学报,2007,35(8):66-67,70. |
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| 作者姓名： | 惠丰立 冯金荣 杨柯金 文祯中 |
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| 作者单位： | 南阳师范学院,南阳,473061 |
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| 基金项目： | 河南省自然科学基金项目(0411032300)。 |
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| 摘 要： | 从番茄果实表面筛选到1株产几丁质酶酵母菌株CY-6,该菌株产生的几丁质酶对番茄镰刀菌果腐病菌(Fusarium oxysporum)有较强的抗菌活性并具有较广的抗菌谱。形态及生理生化特征测定结果表明:菌株CY-6与伊萨酵母属(Issatchenkia)中的陆生伊萨酵母(I.terricola)种的特征基本一致;测定了该菌株的26S rDNAD1/D2区域序列并根据26S rDNA构建了系统发育树;在系统发育树中,菌株CY-6与陆生伊萨酵母形成一个类群,序列同源性高达99.6%。因此,将菌株CY-6鉴定为陆生伊萨酵母。
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| 关 键 词： | 菌株CY-6 几丁质酶 26S rDNA 系统发育 |
| 修稿时间： | 2006-10-17 |
Screening and Identification of Chitinase-Producing Yeast |
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| Hui Fengli,Feng Jinrong,Yang Kejin,Wen Zhenzhong.Screening and Identification of Chitinase-Producing Yeast[J].Journal of Northeast Forestry University,2007,35(8):66-67,70. |
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| Authors: | Hui Fengli Feng Jinrong Yang Kejin Wen Zhenzhong |
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| Institution: | College of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China |
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| Abstract: | A chitinase-producing yeast isolate CY-6 was screened from the surface of tomato fruits. The chitinase produced by strain CY-6 could strongly inhibit the growth of Fusarium oxysporum and exhibited inhibition against a wide-range of plant pathogenic fungi. Morphological, physiological and biochemical characteristics of strain CY-6 showed that the characteristics of strain CY-6 were essentially consistent with those of Issatchenkia terricola. The analysis of 26S rDNAD1/D2 domain sequence from strain CY-6 suggested that strain CY-6 was clustered together with I. terricola in the phylogenetic tree, and the sequence identity between strain CY-6 and I. terricola was 99.6%. The result indicates that strain CY-6 belongs to I. terricola. |
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| Keywords: | Yeast CY-6 Chitinase 26S rDNA Phylogenetic analysis |
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