| PLIN1第2、4外显子多态性与猪脂肪沉积性状的相关性分析 |
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| 引用本文： | 雷娜,贾青,赵广珍,张翠翠,冯安学,邢增喜.PLIN1第2、4外显子多态性与猪脂肪沉积性状的相关性分析[J].河北农业大学学报,2012,35(3):106-111. |
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| 作者姓名： | 雷娜 贾青 赵广珍 张翠翠 冯安学 邢增喜 |
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| 作者单位： | 1. 河北农业大学动物科技学院,河北保定,071000
2. 河北农业大学动物科技学院,河北保定071000;国家北方山区农业工程技术研究中心,河北保定071000 |
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| 摘 要： | 为探索编码脂滴包被蛋白的PLIN1基因对猪脂肪沉积的作用,随机选择190头杜×长×大三元商品杂交猪屠宰,采用常规方法测定部分脂肪沉积性状,同时采集样品提取DNA；运用PCR-SSCP技术对猪PLIN1基因第2、4外显子进行SNP筛查；采用一般线性模型分析所得基因型对各脂肪沉积性状的影响作用.在猪PLIN1基因第2、4外显子分别发现一处碱基替换突变.第2外显子的AA型背膘厚高于AB和BB型(P＜0.01),AA型剪切力值低于AB型和BB型(P＜0.01)；第4外显子的CD型背膘厚高于CC型(P＜0.05),CD型肌内脂肪含量高于CC型(P＜0.01).结果表明,PLIN1基因的第2、4外显子SNP对猪脂肪沉积相关性状有显著作用.
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| 关 键 词： | 猪 PLIN1基因 PCR-SSCP 脂肪沉积 |
Correlation analysis between SNPs of PLIN1 Exon 2,4 and swine fat deposition traits |
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| LEI Na
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JIA Qing
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ZHAO Guang-zhen
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ZHANG Cui-cui
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FENG An-xue
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XING Zeng-xi.Correlation analysis between SNPs of PLIN1 Exon 2,4 and swine fat deposition traits[J].Journal of Agricultural University of Hebei,2012,35(3):106-111. |
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| Authors: | LEI Na JIA Qing ZHAO Guang-zhen ZHANG Cui-cui FENG An-xue XING Zeng-xi |
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| Institution: | 1(1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China; 2.National Engineering Research Center for Agriculture in Northern Mountainous Area,Baoding 071000,China) |
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| Abstract: | To explore the effect of PLIN1 which encoding Perilipin on swine fat deposition traits,190 Duroc × Landrace ×Large White hybrid pigs were randomly selected and slaughtered.Fat deposition traits were measured by routine methods and meat samples were collected for DNA extraction.PCR-SSCP method was used for SNP screening on the exon 2 and exon 4 of swine PLIN1 gene.Effect of the genotype on fat deposition traits was analyzed based on the general linear model.Two base substitutions were found in the exon 2 and exon 4,respectively.AA type of exon 2 was higher than AB and BB type in back fat thickness(P<0.01),but lower in the shear force measurements(P<0.01).CD type of exon 4 was higher than CC type in both of the back fat thickness(P<0.05) and the Intramuscular fat content(IMF)(P<0.01). The results indicated that the SNP of exon 2 and exon 4 of PLIN1 had significant effects on swine fat deposition traits. |
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| Keywords: | swine PLIN1 gene PCR-SSCP fat deposition |
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