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峨眉含笑基因组DNA提取及RAPD反应体系的优化
引用本文:朱秀志,向成华,彭正松,杨俊宝,罗成科.峨眉含笑基因组DNA提取及RAPD反应体系的优化[J].河北农业大学学报,2006,29(2):46-49.
作者姓名:朱秀志  向成华  彭正松  杨俊宝  罗成科
作者单位:1. 西华师范大学,生命科学院,四川,南充,637002
2. 四川林业科学研究院,四川,成都,610000
3. 宁夏大学,宁夏,银川,750021
摘    要:以峨眉含笑嫩叶为材料,研究了峨眉含笑基因组DNA提取方法及RAPD反应条件。结果表明:采用改良的CTAB方法,提取的DNA质量较高,适宜于RAPD-PCR分析。在25μL反应体积中,RAPD分析的优化反应体系为:1.5 mmol/L Mg2+、0.8 mmol/L dNTP、240 nmol/L随机引物、80 ng DNA、1.0 U Taq DNA聚合酶。

关 键 词:峨眉含笑  DNA提取  RAPD  优化
文章编号:1000-1573(2006)02-0046-04
收稿时间:2005-10-21
修稿时间:2005-10-21

Genomic DNA extraction and optimization of RAPD analytic conditions of Michelia wilsonii
ZHU Xiu-zhi,XIANG Cheng-hua,PENG Zheng-song,YANG Jun-bao,LUO Cheng-ke.Genomic DNA extraction and optimization of RAPD analytic conditions of Michelia wilsonii[J].Journal of Agricultural University of Hebei,2006,29(2):46-49.
Authors:ZHU Xiu-zhi  XIANG Cheng-hua  PENG Zheng-song  YANG Jun-bao  LUO Cheng-ke
Institution:1. College of Life Science, China West Normal University, Nanchong 637002, China; 2. Sichuan Academy of Forestry, Chengdu 610000, China; 3. Ningxia University, Yinchuan 750021, China
Abstract:The method of genomic DNA extraction and the optimization of RAPD analytic conditions were studied in Michelia wilsonii.The results showed that high grade genomic DNA was obtained by the revised CTAB method.The optimal PCR system for RAPD analysis was as follows: 1.5 mmol/L Mg~(2+),0.8 mmol/L dNTP,240 nmol/L random primer,80 ng DNA template,1.0 U Taq polymerase,in 25 μL reaction system.
Keywords:Michelia wilsonii  DNA extraction  RAPD  optimization
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