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| 青蒿高产株系001法呢基焦磷酸合酶基因的克隆、原核表达及酶活性测定 | |
| 引用本文： | 韩军丽,李振秋,叶和春,李国凤.青蒿高产株系001法呢基焦磷酸合酶基因的克隆、原核表达及酶活性测定[J].河北农业大学学报,2008,31(4). |
| 作者姓名： | 韩军丽 李振秋 叶和春 李国凤 |
| 作者单位： | 1. 天津市食品生物技术重点实验室,天津商业大学,生物技术与食品科学学院,天津,300134;中国科学院植物研究所,光合作用与环境分子生理学重点实验室,北京,100093 2. 中国科学院植物研究所,光合作用与环境分子生理学重点实验室,北京,100093;河北大学,生命科学学院,河北,保定071002 3. 中国科学院植物研究所,光合作用与环境分子生理学重点实验室,北京,100093 |
| 基金项目： | 天津商业大学引进人才科研启动项目 |
| 摘 要： | 法呢基焦磷酸合酶(FPS)是治疗疟疾的特效药物——青蒿素生物合成途径的关键酶。运用RT-PCR方法从青蒿高产株系001中克隆法呢基焦磷酸合酶基因;构建His标签融合蛋白FPS表达载体,进行原核诱导表达、蛋白纯化及酶活性测定的研究。结果表明:克隆的FPS基因和文献已报道的2个青蒿FPS基因编码的氨基酸序列的同源性分别为98.83%和99.42%;原核诱导表达的His标签融合蛋白FPS的表达量高、可溶性好,具有FPS酶活性。 |
| 关 键 词： | 法呢基焦磷酸合酶(FPS) 青蒿(Artemisia annua L．) 基因克隆 原核表达 蛋白纯化 |
Molecular cloning, prokaryotic expression,and enzyme activity assay of fps from Artemisia annua strain 001 |
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| HAN Jun-li,LI Zhen-qiu,YE He-chun,LI Guo-feng.Molecular cloning, prokaryotic expression,and enzyme activity assay of fps from Artemisia annua strain 001[J].Journal of Agricultural University of Hebei,2008,31(4). | |
| Authors: | HAN Jun-li LI Zhen-qiu YE He-chun LI Guo-feng |
| Abstract: | Farnesyl diphosphate synthase(FPS)is a key enzyme in the biosynthetic pathway of arte- misinin,a new effective antimalarial drug.In this work,the fps was cloned from Artemisia annua strain 001 by RT-PCR; prokaryotic expression of FPS,purification,and enzyme activity assay were carried out.The results indicated that the homology of amino acid deduced from this FPS gene was 98.83% and 99.42% respectively to other two reported Artemisia annua fps; the His-tag FPS was expressed with high level,high solubility,and FPS enzyme activity. |
| Keywords: | Farnesyl diphosphate synthase(FPS) Artemisia annua L cloning prokaryotic expression protein purification |
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