| 嫁接技术在大豆花叶病毒长距离运输机制研究上的应用 |
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| 引用本文： | 姚贵滨,李文龙,赵永山,郑文永,王冬梅. 嫁接技术在大豆花叶病毒长距离运输机制研究上的应用[J]. 河北农业大学学报, 2010, 33(6) |
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| 作者姓名： | 姚贵滨 李文龙 赵永山 郑文永 王冬梅 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金资助(30971706); 河北省自然科学基金资助(C2008000321) |
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| 摘 要： | 大豆花叶病毒侵染大豆植株后,通过胞间连丝进行胞间运输,通过维管束系统完成长距离运输。传统的摩擦接种只适合研究胞间运输,在研究大豆花叶病毒长距离运输方面,目前还没有一个较好的方法能够将大豆花叶病毒接种到维管束。本试验通过大豆嫁接过程中苗期的选择、切口长度以及切口的处理等条件进行了摸索,利用嫁接技术将病毒接种到维管束,为研究病毒的长距离运输机制提供技术保障。
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| 关 键 词： | 嫁接 大豆花叶病毒 CP |
The application of graft technique in the soybean for investigation of soybean mosaic virus transporting mechanism |
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| YAO Gui-bin,LI Wen-long,ZHAO Yong-shan,ZHENG Wen-yong,WANG Dong-mei. The application of graft technique in the soybean for investigation of soybean mosaic virus transporting mechanism[J]. Journal of Agricultural University of Hebei, 2010, 33(6) |
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| Authors: | YAO Gui-bin LI Wen-long ZHAO Yong-shan ZHENG Wen-yong WANG Dong-mei |
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| Affiliation: | YAO Gui-bin,LI Wen-long,ZHAO Yong-shan,ZHENG Wen-yong,WANG Dong-mei(College of Life Science,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | After infected the plants of soybean,soybean mosaic virus(SMV) will be transported between the cell through the plasmodesmata and complete the long-distance transported through the vascular bundle.The traditional mechanical inoculation,friction inoculation,is only applicable to study cell-to-cell movement.Nowadays,in the study area of virus long-distance transport,there is non of a better way to let the SMV inoculate to the vascular.This paper will explore the conditions of the seedling selection,length of ... |
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| Keywords: | graft soybean mosaic virus coat protein |
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