| 蹄甲角蛋白酶高产菌株的紫外诱变及酶学性质研究 |
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| 引用本文： | 丁淑丽,郭润芳,于宏伟,贾英民.蹄甲角蛋白酶高产菌株的紫外诱变及酶学性质研究[J].河北农业大学学报,2012,35(2):79-82. |
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| 作者姓名： | 丁淑丽 郭润芳 于宏伟 贾英民 |
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| 作者单位： | 1. 河北农业大学食品科技学院,河北保定,071001
2. 河北农业大学食品科技学院,河北保定071001;河北科技大学生物科学与工程学院,河北石家庄050018 |
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| 基金项目： | 河北省科技支撑计划项目，河北省教育厅重点项目 |
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| 摘 要： | 为了得到蹄甲角蛋白酶高产菌株,本试验对1株产角蛋白酶的黄杆菌(Chryseobacteriumsp.)N5菌株进行紫外诱变,筛选得到高产突变株U3-22,利用考马斯亮蓝法测定U3-22菌株的角蛋白酶发酵活力达69.9U/mL,比出发菌株的25.4U/mL提高了2.75倍。该角蛋白酶最适反应温度是70℃,最适pH是7.5;K+、Mg2+、Na2SO3对该角蛋白酶有较明显的促进作用,而Mn2+、Zn2+的抑制作用较为明显;突变株U3-22产生的角蛋白酶对蹄甲粉具有很强的分解能力,且具有较好的热稳定性和pH稳定性。结果表明,U3-22产生的角蛋白酶是一种新型的耐高温、耐酸碱角蛋白酶,在动物蹄甲、羽毛等废弃资源的利用中有巨大的应用前景。
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| 关 键 词： | 蹄甲 角蛋白酶 紫外诱变 酶学性质 |
The UV mutagenesis of a high-yielding hoof keratinase strain and enzymatic characteristics |
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| DING Shu-li
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GUO Run-fang
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YU Hong-wei
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JIA Ying-min.The UV mutagenesis of a high-yielding hoof keratinase strain and enzymatic characteristics[J].Journal of Agricultural University of Hebei,2012,35(2):79-82. |
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| Authors: | DING Shu-li GUO Run-fang YU Hong-wei JIA Ying-min |
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| Institution: | 1,2(1.College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China; 2.College of Bioscience and Engineering,Science and Technology University of Hebei,Shijiazhuang 050018,China) |
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| Abstract: | In this paper,the high-yield hoof keratinase strain U3-22 was obtained by UV mutagenesis on the original strain N5.The hoof keratinase production reached 69.9 U/mL using the method of coomassie brilliant blue colorimetric,which was 2.75 times higher than that of the original strain with 25.4 U/mL.The enzymatic characteristics showed that the optimum reaction temperature and pH were 70 ℃ and 7.5,respectively.The enzyme activity could be clearly activated by K+,Mg2+ and Na2SO3,and inhibited by Mn2+ and Zn2+.The keratinase could degrade the hoof effectively.These results suggest that the keratinase from the mutant strain U3-22 is novel,thermal stable and pH-tolerant,and it can be applied in waste disposal of hoof and feather. |
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| Keywords: | hoof keratinase UV mutagenesis enzymatic characteristics |
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