双链RNA分析技术在鉴定RNA病毒中的应用

ｄｓＲＮＡ技术是以无ＲＮＡ病毒或类似病毒因子侵染的植物组织中不含有容易鉴定的同原大分子的ｄｓＲＮＡ（＞０．１×１０６）存在为前提。在植物体内ｄｓＲＮＡ是ＲＮＡ病毒和类病毒因子存在的迹象，ｄｓＲＮＡ包括ｄｓＲＮＡ病毒的基因或ｓｓＲＮＡ病的复制中间型。ｄｓＲＮＡ在寄主组织相当稳定，可以用物理和化学的方法分离出来。叙述了ｄｓＲＮＡ技术用于植物病毒群、成员、同一病毒的不同株系、感染作物的新病毒及其复合侵染、类病毒、卫星病毒等的鉴定。证明该方法使用广泛、快速准确，不需要贵重设备，在一般实验室中即可进行。该方法有重要应用和学术价值，已引起植物病毒和植物病理学工作者广泛兴趣。

Applications of dsRNA Analysis to The detection of RNA Viruses

The principle and techniqes of dsRNA analysis was summarized for the identification of RNA viruses. The dsRNA approach is based on the premise that plants not infected with RNA viruses or virus-like agents do not Contain readily detectable amount of homogeneous Segements of high molecular Weight (>0.1×106) dsRNA. accepting this premise, the presence of dsRNA in a plant is a footprint of an RNA virus or Virus-like agents. The dsRNA detected could be the genome of a dsRNA virus or the replicative dsRNA of a single-stranded RNA (ssRNA) virus. the dsRNA quite stable and can usually be isolated by physical or chemical method. Techniques for the isolation, purification and analysis of dsRNA were described.Applications of dsRNA analysis to the defection of plant viruses,the 19 groups of plant viruses、 cryptic virus、 viruses of a crop, the 26 solates of CMV, Satellites RNA. virold, etc. were identified by dsRNA analysis.