|
|||||
|
|
| 苹果砧木耐盐突变体的筛选鉴定及RAPD分析 | |
| 引用本文： | 孙宁,孙建设,李增裕.苹果砧木耐盐突变体的筛选鉴定及RAPD分析[J].河北农业大学学报,2004,27(5):37-40. |
| 作者姓名： | 孙宁 孙建设 李增裕 |
| 作者单位： | 1. 天津农学院,农学系,天津,300384 2. 河北农业大学,园艺学院,河北,保定,071001 3. 中国农业部,种植业管理司,北京,100026 |
| 基金项目： | 河北农业大学9816项目资助 |
| 摘 要： | 对由诱变获得的苹果砧木78-48耐盐变异细胞系再生植株进行多代盐筛选,获得耐盐性稳定的突变体。并对突变体进行了RAPD分析,153个引物中有36个引物扩增出多态性,证明突变体在基因上发生了变化,为耐盐突变体的真实性提供了证据。 |
| 关 键 词： | 苹果砧木 突变体 耐盐筛选 RAPD |
| 文章编号： | 1000-1573(2004)05-0037-04 |
Salt tolerant mutant screening and RAPD analysis studies on apple rootstock |
|
| SUN Ning,SUN Jian-she,LI Zeng-yu.Salt tolerant mutant screening and RAPD analysis studies on apple rootstock[J].Journal of Agricultural University of Hebei,2004,27(5):37-40. | |
| Authors: | SUN Ning SUN Jian-she LI Zeng-yu |
| Institution: | SUN Ning~1,SUN Jian-she~2,LI Zeng-yu~3 |
| Abstract: | Mutants were regenerated from salt tolerant cell line of apple rootstock. The mutants were screened by salt stress and the salt tolerant mutants were obtained. Through RAPD (Random Amplified Polymorphic DNA) analysis of rootstock and it's mutants, thirty-six out of 153 random primers could detect DNA polymorphism. Results showed the mutants varied not only on physiologic level, but also on the DNA level, which supplied hard evidence of the truth of the mutants and ruled out the possibility of salt adaptation. |
| Keywords: | apple rootstock salt tolerant mutants salt stress RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |