拟南芥磷酸肌醇特异性磷脂酶CAtPLC15亚型基因的组织表达定位

采用PCR扩增的方法,得到AtPLC151 380 bp的启动子片段,构建了带动报告基因GUS表达的双元载体,转化拟南芥并筛选阳性转基因植株。通过组织化学染色分析AtPLC15基因在拟南芥中的表达情况,结果显示AtPLC15基因在拟南芥幼苗的子叶、下胚轴、根尖和柱头处有表达,在成熟花药中表达量极少,而在幼苗子叶和根尖中有极高的表达量。对该基因的功能有了新的认识,为阐述其功能机理提供了重要研究基础。

Expression and localization of AtPLC15 a gene encoding a phosphatidylinositol-specific phospholipase C in Arabidopsis thaliana

A pair of specific primer was designed according to the published sequence of the 5′up stream sequence of the promoter of AtPLC15 in Arabidopsis thaliana.A 1 380 bp sequence was amplified from genomic DNA template of Arabidopsis thaliana by PCR.The cloned promoter has been used in construction of grobacterium binary vectors with GUS reporter gene,and several transformed plants were obtained through a grobacterium-mediated transformation.GUS activity in various tissues of transformed plants was examined and the results showed that GUS gene directed by the promoter sequence of AtPLC15 was tissue-specifically expressed in cotyledon,hypocotyls,root tip,pollon and chapiter.And in semet its expression level is low while in seed leaf and root tip it has a high level.This experiment has led to a novel recognition of the said gene,and thereby providing a foundation for further experiment of its functional mechanism.