| 灵芝倍半萜合酶基因的克隆及表达 |
| |
| 引用本文： | 王丽芝,浦香东,谭世强,孙思杰,毕钰彤,孙超,陈士林,王海英.灵芝倍半萜合酶基因的克隆及表达[J].河北农业大学学报,2017,40(2). |
| |
| 作者姓名： | 王丽芝 浦香东 谭世强 孙思杰 毕钰彤 孙超 陈士林 王海英 |
| |
| 作者单位： | 1. 天津中医药大学 中药学院,天津,300193;2. 天津中医药大学 实验教学部,天津,300193;3. 中国医学科学院 北京协和医学院药用植物研究所,北京,100193 |
| |
| 基金项目： | 国家自然科学基金，天津市自然科学基金，天津市高等学校科技发展基金，大学生创新创业训练计划项目 |
| |
| 摘 要： | 倍半萜合酶是倍半萜化合物生物合成途径中的关键酶。本试验从灵芝基因组数据库中筛选出1个编码灵芝萜类合酶的基因,并对其进行克隆及表达。序列分析表明,该基因开放阅读框的长度为1041bp,编码346个氨基酸;成功构建了1个灵芝萜类合酶基因的原核表达载体,并完成了该基因编码的倍半萜合酶在大肠杆菌中的可溶性表达;顶空固相微萃取—气相色谱质谱法分析结果表明重组的灵芝萜类合酶具有催化活性。为后续研究萜类化合物的多样性奠定基础。
|
| 关 键 词： | 灵芝 倍半萜合酶 生物合成 |
Cloning and expression of the sesquiterpene synthase gene from Ganoderma lucidum |
| |
| WANG Li-zhi,PU Xiang-dong,TAN Shi-qiang,SUN Si-jie,BI Yu-tong,SUN Chao,CHEN Shi-lin,WANG Hai-ying.Cloning and expression of the sesquiterpene synthase gene from Ganoderma lucidum[J].Journal of Agricultural University of Hebei,2017,40(2). |
| |
| Authors: | WANG Li-zhi PU Xiang-dong TAN Shi-qiang SUN Si-jie BI Yu-tong SUN Chao CHEN Shi-lin WANG Hai-ying |
| |
| Abstract: | Sesquiterpene synthases are key enzymes involved in the biosynthetic pathway of sesquiterpenoids.In this study,the candidate gene encoding a sesquiterpene synthase was obtained from Ganoderma lucidum genome database,and then was cloned and expressed in E.coli.The result of sequence analysis showed that the gene contained a complete open reading frame(ORF)with 1041 bp,encoding 346 amino acids.A prokaryotic expression vector of the sesquiterpene synthase was successfully constructed,and the recombinant sesquiterpene synthase was successfully expressed in E.coli BL 21.The result of solid-phase micro-extraction and GC-MS (SPME-GC-MS) revealed that the recombinant sesquiterpene synthase had the catalytic activity.These results laid foundation for the follow-up investigation of sesquiterpenoids diversity. |
| |
| Keywords: | Ganoderma lucidum sesquiterpene synthase biosynthesis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
