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SSR和ISSR分子标记及其在植物遗传育种研究中的应用
引用本文:张立荣,徐大庆,刘大群.SSR和ISSR分子标记及其在植物遗传育种研究中的应用[J].河北农业大学学报,2002,25(1):90-94.
作者姓名:张立荣  徐大庆  刘大群
作者单位:河北农业大学植保学院,河北保定,071001
基金项目:河北省自然科学基金资助项目 (3970 99)
摘    要:SSR(Simple Sequence Repeat)和ISSR(Inter-Simple Sequence Repeat)技术是在PCR基础上发展起来的两种DNA多态性检测技术,已开始应用于基因组研究的各个领域。概述了SSR、ISSR反应的原理、特点,总结了其在植物亲缘关系和遗传多态性研究、DNA指纹库的建立、遗传图谱的构建和基因定位及分子标记辅助育种等方面的应用,并肯定了SSR、ISSR在植物遗传育种领域的广阔应用前景。

关 键 词:植物  遗传育种  应用  SSR  ISSR  分子标记
文章编号:1000-1573(2002)01-0090-05
修稿时间:2001年3月22日

SSR marker, ISSR marker and their application to plant genetics and breeding
ZHANG Li rong,XU Da qing,LIU Da qun.SSR marker, ISSR marker and their application to plant genetics and breeding[J].Journal of Agricultural University of Hebei,2002,25(1):90-94.
Authors:ZHANG Li rong  XU Da qing  LIU Da qun
Abstract:Simple sequence repeats (SSR) and Inter-simple sequence repeats (ISSR) are two kinds of DNA markers based on the polymerase chain reaction (PCR). They have been applied in many aspects of genome research. This paper has clarified the theories and characteristics of SSR as well as ISSR. The authers also summarized their applications in genetic polymorphisim and genetic relationship, establishment of DNA fingerprinting pool, construction of genetic map, gene localization and marker-aided selection. The two methods of DNA ecular marker open up broad prospects for the studies of plant genetics and breeding.
Keywords:SSR  ISSR  molecular marker  genetics breeding
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